Search for dissertations about: "15N and 13C NMR"

Showing result 1 - 5 of 16 swedish dissertations containing the words 15N and 13C NMR.

  1. 1. Molecular recognition and dynamics in proteins studied by NMR

    Author : Johan Wallerstein; Biofysikalisk kemi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Chemical exchange; Conformational entropy; Entropy; Galectin-3; Isothermal Titration Calorimetry; ITC; Model-free formalism; Molecular recognition; Nuclear Magnetic Resonance; NMR; PGB1; Protein dynamics; Protein–ligand interactions; Proton-transfer reactions; Spin relaxation; Viscosity; Chemical exchange; Conformational entropy; Entropy; Galectin-3; Isothermal Titration Calorimetry; ITC; Model-free formalism; Molecular recognition; Nuclear Magnetic Resonance; NMR; PGB1; Protein dynamics; Protein–ligand interactions; Proton-transfer reactions; Spin relaxation; Viscosity;

    Abstract : Knowledge of dynamics in protein is very important in the description of protein function and molecular recognition. The thesis investigates protein dynamics on time-scales from milli- to sub-nanosecond, with focus on the latter, using NMR spin relaxation experiments on two proteins, the 138-residue carbohydrate recognition domain of galectin-3 (Gal3C) and the 56-residue B1 domain of bacterial protein G (PGB1). READ MORE

  2. 2. Structure and function studies of GH45 glycoside hydrolases

    Author : Laura Okmane; Sveriges lantbruksuniversitet; Sveriges lantbruksuniversitet; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES;

    Abstract : The enzymatic hydrolysis of lignocellulosic material in nature is carried out by a plethora of cellulases. Glycoside hydrolase family 45 (GH45) enzymes are small cellulases most commonly found in fungi that catalyse the hydrolysis of β(1→4) linked glucans. READ MORE

  3. 3. Characterizing Chromatography Media : NMR-based Approaches

    Author : Fredrik Elwinger; István Furó; Stéphane Viel; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; chromatography; nuclear magnetic resonance; NMR; self-diffusion; magic-angle spinning; quantitative; distribution coefficient; pore size; Kemi; Chemistry;

    Abstract : Liquid chromatography is an essential technique in manufacturing biopharmaceuticals where it is used on all scales from analytical applications in R&D to full-scale production. In chromatography the target molecule, typically a protein, is separated and purified from other components and contaminants. READ MORE

  4. 4. Structural and functional studies of biomolecules with NMR and CD spectroscopy

    Author : Evangelos Papadopoulos; Astrid Gräslund; Göran Karlsson; Stockholms universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NMR; protein structure; nouse Doppel; HTH; C-repressor; potassium channel; Biophysics; Biofysik; Biophysics; biofysik;

    Abstract : Experimentally derived biomolecular structures were determined by Nuclear Magnetic Resonance (NMR). The properties of selected peptides and proteins in solution and in membrane mimicking micelles were observed by circular Dichroism (CD), mass spectrometry (MS), and other spectroscopic techniques. READ MORE

  5. 5. Structure and Dynamics of the Hepatitis B Virus Encapsidation Signal Revealed by NMR Spectroscopy

    Author : Sara Flodell; Stephan Grzesiek; Umeå universitet; []
    Keywords : HBV; RNA; isotope labelling; NMR; structure determination; medicinsk biokemi; Medical Biochemistry;

    Abstract : This thesis describes the study of the three-dimensional structure and dynamics of the hepatitis B virus (HBV) encapsidation signal, epsilon, by means of nuclear magnetic resonance (NMR) and mutational data. HBV replicates by reverse transcription of an RNA pregenome into the viral DNA genome, which becomes enclosed in viral particles (encapsidation). READ MORE