Search for dissertations about: "32P-HPLC"

Found 5 swedish dissertations containing the word 32P-HPLC.

  2. 1. UV-induced DNA damage in humans

    Author : Vladimir J Bykov; Karolinska Institutet; Karolinska Institutet; []
    Keywords : 32P-HPLC; photoproducts; DNA repair; solar simulated radiation; erythema; sunscreens; humans;

    Abstract : Ultraviolet radiation is considered to be the most harmful part of solar energy affecting man. The depletion of the ozone layer around the Earth increases the total exposure to UV-light. The incidence of skin cancer in man has been shown to be associated with exposure to solar radiation, especially to UV-light. READ MORE

  4. 2. High-performance liquid chromatography for analysis of 32P-postlabeled DNA adducts

    Author : Magnus Zeisig; Karolinska Institutet; Karolinska Institutet; []
    Keywords : DNA adducts; 32P-postlabeling; HPLC; method development;

    Abstract : The formation of DNA adducts, i.e. the covalent binding of chemicals and chemical groups to DNA, is believed to be an important step in chemical carciogenesis. DNA adducts are usually formed at very low levels which requires very sensitive methods to analyze them. READ MORE

  5. 3. Method development for analysis of 8-oxodG as a biomarker for oxidative stress

    Author : Tim Hofer; Karolinska Institutet; Karolinska Institutet; []
    Keywords : ;

    Abstract : Oxidative damage to DNA gives oxidation of DNA bases and strand breaks that can cause mutations leading to cancer. Various forms of agents are capable of oxidising DNA in vivo including peroxides, singlet oxygen and UV- or gamma-irradiation. Oxidation of DNA bases occurs mainly at dG, giving stable DNA adducts such as 8-oxodG and FapydG. READ MORE

  6. 4. DNA lesions and carcinogenicity from the urban air pollutants 2- and 3-nitrobenzanthrone

    Author : Eszter Nagy; Karolinska Institutet; Karolinska Institutet; []
    Keywords : 3-nitrobenzanthrone; 2-nitrobenzanthrone; diesel exhaust; carcinogenicity; acute toxicity; DNA adducts; oxidative stress; intratracheal; ingestion;

    Abstract : In the early 1990's, Japanese doctors in urban areas discovered an increasing incidence of lung cancer among women, which was not attributed to a change in diet or smoking. This led to a growing investigation of urban air, and subsequently the discovery of 3-nitrobenzanthrone (3-NBA), a pollutant originating from diesel emissions. READ MORE

  7. 5. Studies on DNA adducts and carcinogenesis with 2-nitrofluorene as a model substance

    Author : Xian-Shu Cui; Karolinska Institutet; Karolinska Institutet; []
    Keywords : 2-nitrofluorene; carcinogens; DNA adducts; preneoplastic lesion; tumor;

    Abstract : A number of carcinogens or their electrophilic intermediates are able to bind covalently to DNA, forming DNA adducts. DNA adducts may cause gene mutations if they are misrepaired or remain unrepaired before DNA replication occurs. DNA adduct formation is believed to be the initial step of chemical carcinogenesis. READ MORE