Search for dissertations about: "Affinity Partitioning"
Showing result 1 - 5 of 20 swedish dissertations containing the words Affinity Partitioning.
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1. Isolation of caveolae using affinity two-phase partitioning
Abstract : The intention of this work was to establish alternative purification methods to obtain highly purified caveolae from various tissues. In order to isolate caveolae, sufficiently pure plasma membranes are needed. READ MORE
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2. Affinity-, Partition- and Permeability Properties of the Human Red Blood Cell Membrane and Biomembrane Models, with Emphasis on the GLUT1 Glucose Transporter
Abstract : The human glucose transporter GLUT1 is abundant in red blood cells, the blood-brain barrier and epithelial cells, where it mediates the transport of the energy metabolite, glucose. In the present work some properties of GLUT1, including affinity binding of both substrates and inhibitors, transport rates as well as permeabilities of aromatic amino acids and drug-membrane interactions were analyzed by chromatographic methods. READ MORE
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3. Chromatographic Studies of Solute Interactions with Immobilized Red Blood Cells and Biomembranes
Abstract : Specific and non-specific interactions of solutes with immobilized biomembranes were studied using chromatographic methods. Liposomes, proteoliposomes and red blood cell (RBC) membrane vesicles were immobilized by a freeze-thawing procedure, whereas whole RBCs were adsorbed in the gel beds using electrostatic interaction, binding to wheat germ agglutinin (WGA) or the streptavidin-biotin interaction. READ MORE
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4. Selective extraction systems for detection and purification of membrane proteins
Abstract : Increased understanding of membrane proteins is important for characterization of biochemical processes. Improved membrane protein isolation methods is a key issue for effective functional and structural determination of the large amount of unknown membrane proteins. READ MORE
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5. Affinity Two-Phase Partitioning of Liposomes and Membranes
Abstract : Affinity two-phase partitioning based on an immunoaffinity sandwich approach for the rapid and selective purification of membranes is presented in this thesis. The method was developed by studying different parameters governing the affinity partitioning of model membranes. To this end, biotinylated liposomes were used. READ MORE