Search for dissertations about: "Amide hydrogen exchange"

Showing result 1 - 5 of 12 swedish dissertations containing the words Amide hydrogen exchange.

2. 1. Method Development in Quantitative and Structural Proteomics using Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

   Author : Charlotte Hagman; Per Håkansson; Jonas Bergquist; Jos Buijs; Michael Przybylski; Uppsala universitet; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Biotechnology; Cerebrospinal Fluid; Electrospray Ionization; Fourier Transform Ion Cyclotron Resonance Mass Spectrometry; Global Markers; High Performance Liquid Chromatography; Gas-Phase Exchange; Hydrogen Deuterium Exchange; Plasma; Protoemics; Quantification; Structural Elucidation; Bioteknik; Bioengineering; Bioteknik;

   Abstract : In this thesis, methods for studying different aspects of proteomics were developed with Fourier Transform Ion Cyclotron Resonance, (FTICR), mass spectrometry. The FTICR technique provides ultra-high mass resolving power, mass accuracy at sub ppm level and sensitivity in the attomole region. READ MORE

4. 2. Protein-water interactions studied by molecular dynamics simulations

   Author : Filip Persson; Biofysikalisk kemi; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; MD simulations; NMR nuclear magnetic resonance ; MRD; water dynamics; protein dynamics; protein hydration; Amide hydrogen exchange; Internal water; compressibility;

   Abstract : Most proteins have evolved to function optimally in aqueous environments, and the interactions between protein and water therefore play a fundamental role in the stability, dynamics, and function of proteins. Although we understand many details of water, we understand much less about the protein-water interface. READ MORE

5. 3. The Folding Energy Landscape of MerP

   Author : Ann-Christin Brorsson; Bengt-Harald Jonsson; Daniel Otzen; Umeå universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; protein folding and stability; hydrogen exchange; intermediate; partial unfolding; Biokemi; Biochemistry; Biokemi; Biochemistry; biokemi;

   Abstract : This thesis is based on studies, described in four papers, in which the folding energy landscape of MerP was investigated by various techniques. MerP is a water-soluble 72 amino acid protein with a secondary structure consisting of four anti-parallel β-strands and two α-helices on one side of the sheet in the order β1α1β2β3α2β4. READ MORE

6. 4. Method and technique development in peptide and protein mass Spectrometry

   Author : Kristina Håkansson; Uppsala universitet; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Materials science; electrospray ionization; peptide sequencing; Fourier transform ion cyclotron resonance; hydrogen deuterium exchange; protein structure; Materialvetenskap; Materials science; Teknisk materialvetenskap; Molecular Biotechnology; Molekylär bioteknik;

   Abstract : Mechanistic studies and applicability of several methods and techniques for studying peptides and proteins with mass spectrometry are presented. A model for the protein molecular ion formation in explosive matrix-assisted plasma desorption mass spectrometry is proposed, involving a contribution from the chemical energy release during conversion of the explosive, an enhanced protonation through collisions with products from the explosive decay, and electron scavenging by other products. READ MORE

7. 5. New approaches to preparation of macroporous monoliths for use in liquid chromatography

   Author : Anh Mai Nguyen; Knut Irgum; Tatiana B. Tennikova; Umeå universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; monolith; polycondensation; dissolution-precipitation; epoxy-amine; poly-amide; nylon; emulsion polymerization; characterization; protein separation; liquid chromatography; Analytical chemistry; Analytisk kemi; analytisk kemi; Analytical Chemistry;

   Abstract : High performance liquid chromatography (HPLC) is one of the major techniques in separat-ion sciences. Faster separation and higher efficiency are required to meet ever-growing demands. Despite numerous studies and achievements on improving mass transfer in particulate packings discontinuity seems to be the cornerstone drawback in their development. READ MORE