Search for dissertations about: "Bioactivation"
Showing result 1 - 5 of 40 swedish dissertations containing the word Bioactivation.
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1. Role of Multiple Glutathione Transferases in Bioactivation of Thiopurine Prodrugs : Studies of Human Soluble Glutathione Transferases from Alpha, Kappa, Mu, Omega, Pi, Theta, and Zeta Classes
Abstract : A screening method was developed for identification of catalytically active enzymes in combinatorial cDNA libraries of mutated glutathione transferase (GST) derivatives expressed in E. coli. The method is based on spraying monochlorobimane (MCB) directly over bacterial colonies growing on agar. READ MORE
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2. Adrenal Bioactivation and Toxicity of 3-MeSO2-DDE, o,p´-DDD and DMBA Investigated in Tissue Slice Culture
Abstract : I developed a precision-cut adrenal slice culture procedure to investigate cytochrome P450 (CYP) catalysed irreversible binding and adrenocorticolytic effects in human, rodent, and fish adrenal tissue, ex vivo. Autoradiography and radioluminography of exposed tissue slices showed that the potent adrenal toxicant 3-methylsulphonyl-2,2´-bis(4-chlorophenyl)-1,1´-dichloroethene (MeSO2-DDE) causes a selective metabolite binding in zona fasciculata (ZF), which is diminished by the CYP11B1 inhibitor metyrapone. READ MORE
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3. Tissue-Selective Activation and Toxicity of Substituted Dichlorobenzenes : Studies on the Mechanism of Cell Death in the Olfactory Mucosa
Abstract : The nasal passages are constantly exposed to both air- and bloodborne foreign compounds. In particular, the olfactory mucosa is demonstrated to be susceptible to a variety of drugs and chemicals. READ MORE
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4. Adrenocorticolysis Induced by 3-MeSO2-DDE : Mechanisms of Action, Kinetics and Species Differences
Abstract : The DDT metabolite 3-methylsulphonyl-DDE (3-MeSO2-DDE) induces cell death specifically in the adrenal cortex of mice after a cytochrome P45011B1 (CYP11B1)-catalysed bioactivation. This substance is not only an environmental pollutant, but also a suggested lead compound for an improved chemotherapy of adrenocortical carcinoma (ACC). READ MORE
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5. Epoxides as Contact Allergens - Formation, Sensitising Potency and Structure-Activity Relationships
Abstract : Chemicals in our environment with which we have repeated skin contact can cause skin sensitisation (contact allergy). To trigger an immune response a compound (hapten) must be able to penetrate the skin, where it needs to be reactive enough to bind to proteins and form immunogenic complexes that are recognised as foreign. READ MORE