Search for dissertations about: "Cysteine mutagenesis"
Showing result 1 - 5 of 23 swedish dissertations containing the words Cysteine mutagenesis.
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1. Protein Engineering on Azurin. Expression Mutagenesis and Characterisation of Copper Site Mutants
Abstract : Azurin belongs to a family of small blue copper proteins or cupredoxins which participate in electron transfer processes in plants and bacteria. The type 1 copper site in these proteins is characterised by an intense blue colour, a narrow hyperfine coupling in the EPR signal and, generally, a high reduction potential. READ MORE
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2. Microsomal glutathione transferase : catalysis, in vitro mutagenesis and heterologous expression
Abstract : Microsomal glutathione transferase is a membrane-bound member of the glutathione transferases, a family of multifunctional enzymes involved in the cellular detoxification of xenobiotics and reactive endogenous compounds formed during oxidative stress. Rat liver microsomal glutathione transferase is a trimeric protein with a molecular mass of 17. READ MORE
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3. Cystatin C functions in vitro and in vivo. Studies on target enzyme inhibition by cystatin C variants and cystatin C deficient mice
Abstract : Cystatin C variants with Arg8, Leu9 and/or Val10 replaced by Gly residues were produced by site-directed mutagenesis and E. coli expression. READ MORE
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4. Cellular Uptake of Cystatin C. Subcellular localisation and intracellular effects of a secreted cysteine protease inhibitor
Abstract : Cystatin C is a cysteine protease inhibitor, aimed for secretion, as it is produced with a signal peptide. Its target enzymes are thought to be the lysosomal cysteine cathepsins and legumain. READ MORE
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5. Mutational analysis of melanocortin receptors 1 and 5
Abstract : The melanocortin receptor family includes five subtypes, named MC1R to MC5R. Melanocortin receptors are predominantly coupled to second messenger pathway of cyclic AMP through coupling to G-protein. This study is focused on structure-function relationship of MC1R and MC5R. READ MORE