Search for dissertations about: "Esmeralda A. Woestenenk"

Found 1 swedish dissertation containing the words Esmeralda A. Woestenenk.

2. 1. Protein production, characterization and structure determination in structural genomics

   Author : Esmeralda A. Woestenenk; Torleif Härd; Göran Karlsson; KTH; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biology; protein production; protein solubility; fusion tags; nuclear magnetic resonance; structure determination; Biologi; Biology; Biologi;

   Abstract : This thesis covers the process from expression of a heterologous gene in Escherichia coli to structure determination of a protein by nuclear magnetic resonance (NMR) spectroscopy. The first part concerns structural genomics-related parallel screening studies on the effect of fusion tags (in particular the His tag) on protein solubility and the use of fusion tags in fast, parallel purification protocols intended for initial biophysical characterization of human proteins produced in E. READ MORE