Search for dissertations about: "Hydrophobic interaction chromatography"

Showing result 11 - 15 of 27 swedish dissertations containing the words Hydrophobic interaction chromatography.

  2. 11. A radical view of haemoglobin - protein engineering and characterisation

    Author : Marie Grey; Tillämpad biokemi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; His-Gln tagging; protein purification; blood substitutes; radical scavenging; superoxide dismutase; E. coli; diglobin; Haemoglobin; protein engineering; aqueous two-phase systems; hydrophobic interaction chromatography;

    Abstract : In recent years shortages of donated blood have become a growing problem for health care systems world-wide. Blood substitutes based on human haemoglobin (Hb) could potentially ameliorate this situation. However, clinical trials have indicated problems due to e.g. READ MORE

  4. 12. Development and evaluation of analytical techniques for antibodies and antibody-drug conjugates : From verification of conjugation to stability testing

    Author : Malin Källsten; Jonas Bergquist; Fredrik Lehmann; Sara Lind; Lucia Kovac; Alain Beck; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Antibody-drug conjugates; drug-to-antibody ratio; monoclonal antibody; mass spectrometry; hydrophobic interaction chromatography; sample preparation; aggregation; Kemi med inriktning mot analytisk kemi; Chemistry with specialization in Analytical Chemistry;

    Abstract : Antibody-drug conjugates (ADCs) are becoming increasingly important in oncology. ADCs are inherently heterogeneous analytes. For a successful drug candidate to go through to the clinic, extensive characterization and evaluation of the constructs are necessary. READ MORE

  5. 13. Effects of fusion tags on protein partitioning In aqueous two-phase systems and use in primary protein recovery

    Author : Cynthia Hassinen; KTH; []
    Keywords : E. coli; Aqueous two-phase systems; Fusion proteins; Hydrophobic interaction chromatography; Expanded bed adsorption; Beta-galactosidase; Klenow polymerase; Z-domain; Peptide tags;

    Abstract : The two techniques aqueoustwo-phase partitioning and expanded bed adsorption that bothare suitable for primary protein recovery were studied. Most ofthe work was focused on partition in aqueous two-phase systemsand in particular on the possibility to effect the partitionbehaviour by fusion of short peptide tags or protein domains tothe target protein. READ MORE

  6. 14. Effects of Natural Organic Matter on Herbicide Adsorption to Activated Carbon

    Author : Åsa Edell; Chalmers tekniska högskola; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; natural organic matter; herbicide; adsorption isotherm; preloading; activated carbon; competitive interaction;

    Abstract : The presence of pesticides in the aquatic environment represents a potential threat to water quality and human health. Increasingly stringent drinking water quality directives focus on the need for efficient pesticide removal processes. READ MORE

  7. 15. Rational and combinatorial protein engineering for vaccine delivery and drug targeting

    Author : Maria Wikman; Stefan Ståhl; Carl A. K. Borrebaeck; KTH; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Biomedicine; affibody; affinity chromatography; biotin; combinatorial; delivery; phage display; Biomedicin; Microbiology; Mikrobiologi;

    Abstract : This thesis describes recombinant proteins that have been generated by rational and combinatorial protein engineering strategies for use in subunit vaccine delivery and tumor targeting.In a first series of studies, recombinant methods for incorporating immunogens into an adjuvant formulation, e.g. READ MORE