Search for dissertations about: "Immobilized liposome chromatography"

Found 5 swedish dissertations containing the words Immobilized liposome chromatography.

  1. 1. Chromatographic Studies of Solute Interactions with Immobilized Red Blood Cells and Biomembranes

    University dissertation from Uppsala : Acta Universitatis Upsaliensis

    Author : Ingo Gottschalk; Uppsala universitet.; [2002]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Affinity; Binding; Biomembrane; Biotin; Chromatography; Cytochalasin B; Dissociation constant; Drug absorption; Equilibrium; Glucose; GLUT1; Immobilization; Immobilized biomembrane affinity chromatography; Immobilized liposome chromatography; Interaction; Liposome; Membrane protein; Membrane vesicle; Partitioning; Phospholipid bilayer; Proteoliposome; Quantitative; Red blood cell; Solute; Specific; Streptavidin; Wheat germ agglutinin; Biokemi; NATURAL SCIENCES Chemistry Biochemistry; NATURVETENSKAP Kemi Biokemi; Biokemi; Biochemistry;

    Abstract : Specific and non-specific interactions of solutes with immobilized biomembranes were studied using chromatographic methods. Liposomes, proteoliposomes and red blood cell (RBC) membrane vesicles were immobilized by a freeze-thawing procedure, whereas whole RBCs were adsorbed in the gel beds using electrostatic interaction, binding to wheat germ agglutinin (WGA) or the streptavidin-biotin interaction. READ MORE

  2. 2. Affinity-, Partition- and Permeability Properties of the Human Red Blood Cell Membrane and Biomembrane Models, with Emphasis on the GLUT1 Glucose Transporter

    University dissertation from Uppsala : Acta Universitatis Upsaliensis

    Author : Christine Lagerquist Hägglund; Uppsala universitet.; [2003]
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Biochemistry; Affinity; Aromatic amino acids; Binding; Biomembrane; Biotin; Chromatography; Cytochalasin B; Dihydrocytochalasin B; Dissociation constant; Drug absorption; Ethanol; Equilibrium; Glucose; GLUT1; Immobilization; Immobilized biomembrane affinity chromatography; Immobilized liposome chromatography; Interaction; Liposome; Membrane protein; Membrane vesicle; Partitioning; Phospholipid bilayer; Proteoliposome; Quantitative; Red blood cell; Specific; Streptavidin; Tyrosine; Tryptophan; Biokemi; MEDICINE Chemistry Biochemistry; MEDICIN Kemi Biokemi;

    Abstract : The human glucose transporter GLUT1 is abundant in red blood cells, the blood-brain barrier and epithelial cells, where it mediates the transport of the energy metabolite, glucose. In the present work some properties of GLUT1, including affinity binding of both substrates and inhibitors, transport rates as well as permeabilities of aromatic amino acids and drug-membrane interactions were analyzed by chromatographic methods. READ MORE

  3. 3. Drug Partitioning into Natural and Artificial Membranes Data Applicable in Predictions of Drug Absorption

    University dissertation from Uppsala : Institutionen för naturvetenskaplig biokemi

    Author : Caroline Engvall; Uppsala universitet.; [2005]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Bilayer disk; Cholesterol; Drug partitioning; Drugs; Electrostatic effects; Immobilized liposome chromatography; Liposome; Membrane protein; Model membrane; Oligonucleotide-liposome complex; Phospholipid; Phospholipid bilayer; Proteoliposome; Surfactant; Biokemi; NATURAL SCIENCES Chemistry Biochemistry; NATURVETENSKAP Kemi Biokemi;

    Abstract : When drug molecules are passively absorbed through the cell membrane in the small intestine, the first key step is partitioning of the drug into the membrane. Partition data can therefore be used to predict drug absorption. READ MORE

  4. 4. Partitioning of Drugs and Lignin Precursor Models into Artificial Membranes

    University dissertation from Uppsala : Acta Universitatis Upsaliensis

    Author : Elisabet Boija; Uppsala universitet.; [2006]
    Keywords : Biochemistry; Bilayer disk; Capillary electrophoresis; Detergent; Drug; Electrostatic interaction; Hydrophobic interaction; Immobilized liposome chromatography; Lignin; Lignin precursor model; Liposome; Membrane model; Octanol water partitioning; Partitioning; Phospholipid; Phospholipid bilayer; Sterol; Biokemi;

    Abstract : The main aim of this thesis was to characterize membrane-solute interactions using artificial membranes in immobilized liposome chromatography or capillary electrophoresis. The partitioning of a solute into a cell membrane is an essential step in diffusion across the membrane. READ MORE

  5. 5. Partition chromatography of drugs on immobilized liposomes and biomembranes : A method applicable to screening in drug design

    University dissertation from Uppsala : Acta Universitatis Upsaliensis

    Author : Farideh Beigi; Uppsala universitet.; [2000]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Biokemi; NATURAL SCIENCES Chemistry Biochemistry; NATURVETENSKAP Kemi Biokemi; biokemi; Biochemistry;

    Abstract : One of the main pathways for drug passage into cells is diffusion across the lipid bilayer ofthe cell membrane. A key factor in this process is the drug partitioning into the bilayer, whichcan be translated into a retention in a chromatographic system. READ MORE