Search for dissertations about: "Institutionen för naturvetenskaplig biokemi."
Showing result 1 - 5 of 90 swedish dissertations containing the words Institutionen för naturvetenskaplig biokemi..
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1. Structural studies on the integral membrane protein, ubiquinol oxidase from Escherichia coli
Abstract : Heme-copper oxidases are redox-driven proton pumps that couple the reduction of molecular oxygen to water with the vectorial translocation of protons across the membrane. The proton gradient generated by heme-copper oxidases and the other members of the aerobic respiratory chain is ultimately used to drive the synthesis of ATP. READ MORE
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2. Partition chromatography of drugs on immobilized liposomes and biomembranes : A method applicable to screening in drug design
Abstract : One of the main pathways for drug passage into cells is diffusion across the lipid bilayer ofthe cell membrane. A key factor in this process is the drug partitioning into the bilayer, whichcan be translated into a retention in a chromatographic system. READ MORE
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3. Multiple Functions of Glutathione Transferases : A Study on Enzymatic Function, Regulatory Role and Distribution in Mouse and Man
Abstract : To cope with various endogenous toxin and xenobiotics nature has equipped the organisms with a proper protection system. Glutathione transferases (GSTs) are important components of the cellular defense against oxidative stress. These proteins appear to be suited for different tasks. READ MORE
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4. Electrochromatography and liquid chromatography : Microtechniques, instrumentation and applications
Abstract : The thesis focuses on the design of efficient chromatographic and electrochromatographic microcolumns where a continuous bed is polymerized in situ in a capillary or a channel in a microchip device. In our technique, a solution of selected monomers is simply sucked or pressed into the capillary or microchip channel for subsequent polymerization. READ MORE
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5. Redesign of substrate specificity of glutathione transferase and glutathione reductase : Enzyme engineering by directed mutagenesis, phage-display selection and DNA shuffling
Abstract : Human glutathione transferase (GST) A1-1 was expressed in fusion with the phage gene IIIproduct and a library of phage-displayed GST mutants was constructed by randomization of fouractive-site residues. Variant GSTs were isolated by mechanism-based phage display selection, using an immobilized transition-state analog of a nucleophilic aromatic substitution (SNAr) reaction. READ MORE