Search for dissertations about: "Klenow polymerase"

Showing result 1 - 5 of 6 swedish dissertations containing the words Klenow polymerase.

  2. 1. Advancements in Firefly Luciferase-Based Assays and Pyrosequencing Technology

    Author : Jonas Eriksson; KTH; []
    Keywords : bioluminescence; osmolytes; glycine betaine; thermostability; firefly luciferase; inorganic pyrophosphatase; inorganic pyrophosphate; Pyrosequencing technology; secondary DNA-structures; Sequenase; Klenow-polymerase; reaction rates; temperature;

    Abstract : Pyrosequencing is a new DNA sequencing method relying on thesequencing-by-synthesis principle and bioluminometric detectionof nucleotide incorporation events. The objective of thisthesis was improvement of the Pyrosequencing method byincreasing the thermal stability of firefly luciferase, and byintroducing an alternative DNA polymerase and a new nucleotideanalog. READ MORE

  4. 2. Effects of fusion tags on protein partitioning In aqueous two-phase systems and use in primary protein recovery

    Author : Cynthia Hassinen; KTH; []
    Keywords : E. coli; Aqueous two-phase systems; Fusion proteins; Hydrophobic interaction chromatography; Expanded bed adsorption; Beta-galactosidase; Klenow polymerase; Z-domain; Peptide tags;

    Abstract : The two techniques aqueoustwo-phase partitioning and expanded bed adsorption that bothare suitable for primary protein recovery were studied. Most ofthe work was focused on partition in aqueous two-phase systemsand in particular on the possibility to effect the partitionbehaviour by fusion of short peptide tags or protein domains tothe target protein. READ MORE

  5. 3. Protein Engineering by Directed Evolution and Rational Design

    Author : Torbjörn Gräslund; KTH; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; ion-exchange chromatography; subtilisin; Klenow DNA polymerase; Taq DNA polymerase; directed evolution; rational design; charge engineering; phosphonylating inhibitor; phage display; circular dichroism; protein A; Bioengineering; Bioteknik;

    Abstract : .... READ MORE

  6. 4. Mathematical modelling and analysis of the pyrosequencing reaction system

    Author : Anna Svantesson; Jeanette Hellgren Kotaleski; Anders Lansner; Pål Nyrén; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; apyrase; bioluminescence; DNA polymerase; DNA sequencing; enzyme kinetics; Klenow fragment; mathematical modelling; Michaelis-Menten; pyrosequencing; Computer science; Datalogi;

    Abstract : .... READ MORE

  7. 5. Recombinant Enzymes in Pyrosequencing Technology

    Author : Nader Nourizad; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; apyrase; pyrosequencing; pichia pastoris; protein expression; fusion protein; luciferase; DNA template; clone checking; DNA polymerase;

    Abstract : Pyrosequencing is a DNA sequencing method based on thedetection of released pyrophosphate (PPi) during DNA synthesis.In a cascade of enzymatic reactions, visible light isgenerated, which is proportional to the number of nucleotidesincorporated into the DNA template. When dNTP(s) areincorporated into the DNA template, inorganic PPi is released. READ MORE