Search for dissertations about: "Matrix-Assisted Laser Desorption-Ionization"

Showing result 1 - 5 of 51 swedish dissertations containing the words Matrix-Assisted Laser Desorption-Ionization.

  2. 1. Label free analysis and spatial distribution of molecular lipids in brain tissue and cells using mass spectrometry imaging

    Author : Amir Saeid Mohammadi; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Mass Spectrometry Imaging; Secondary Ion Mass Spectrometry Imaging; Matrix Assisted Laser desorption Ionization; Lipids; Brain tissue;

    Abstract : Lipids are important inherent components in brain and cell membranes, which play significant biological roles in cell function. Identification and localization of specific biomolecules such as peptides and lipids within cellular membranes is currently a major challenge in metabolomics and biological studies. READ MORE

  4. 2. Engineering of thermometric biosensors and characterisation of redox enzymes for improved performance of biosensors/biofuel cells

    Author : Maria Yakovleva; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Flow injection analysis; thermometric calorimetric biosensor; enzyme thermistor; immobilised enzyme reactor; amperometric biosensor; enzymatic biofuel cell; bioanode; pyranose dehydrogenase; cellobiose dehydrogenase; direct electron transfer; mediated electron transfer; matrix-assisted laser desorption ionization; glycosite mapping;

    Abstract : The present work is mainly focused on the development of enzyme-based bioanalytical devices, which can further be applied as analytical tools for industrial, medical and environmental applications. The first part of the work deals with the practical applications of thermometric biosensors for determination of various sugars (fructose and lactose) in a real samples. READ MORE

  5. 3. Improved techniques for CE-MALDI-MS off-line coupling and MALDI-MS analysis of primarily hydrophobic proteins and peptides

    Author : Johan Jacksén; Åsa Emmer; Anna Karin/Minna Borg Karlsson/Hakkarainen; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Capillary electrophoresis; Hydrophobic peptides; Matrix-Assisted Laser Desorption Ionization Mass Spectrometry; Prestructured target; Off-line interface; Silicon microcanal; Matrix; Protein cleavage digestion.; Kapillärelektrofores; Hydrofoba peptider; Matris-assisterad laserdesorptions-joniserings-masspektrometri; Strukturbelagd provplatta; Off-line interface; Mikrokanal i kisel; Matris; Proteinklyvning -spjälkning.; Analytical chemistry; Analytisk kemi;

    Abstract : Due to the hydrophobic nature of integral membrane proteins (IMP) they give rise to several difficulties concerning handling and analysis, which is not the case for the most water soluble proteins. New analysis methods are needed, where the insolubility problems of the hydrophobic proteins due to aggregation and adhesion are tackled. READ MORE

  6. 4. Improved techniques for CE and MALDI-MS including microfluidic hyphenations foranalysis of biomolecules

    Author : Johan Jacksén; Åsa Emmer; Tomas Bergman; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Capillary electrophoresis; Hydrophobic peptides proteins; Matrix-Assisted Laser Desorption Ionization Mass Spectrometry; Prestructured target; Off-line interface; Silicon micro canal; Matrix; Peptide-fluorescein isothiocyanate adducts; Bovine serum albumin; DHAP; Hyphenations; Hydrophobicity; Single wood fiber analysis; Pre-concentration; Concentration platform; Analytical chemistry; Analytisk kemi;

    Abstract : In this thesis, improved techniques for biomolecule analysis using capillary electrophoresis (CE) and matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) and hyphenations between those have been presented.A pre-concentration method which is possible to apply in both techniques, has also been investigated. READ MORE

  7. 5. Multimodal Chemical Imaging of Amyloid Plaque Pathology in Alzheimer’s Disease

    Author : Wojciech Michno; Göteborgs universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Alzheimer’s disease; beta-amyloid; electron microscopy; imaging mass spectrometry; lipids; luminescent conjugated oligothiophenes; matrix-assisted laser desorption ionization mass spectrometry MALDI-MS ; molecular imaging; nanoscale secondary ion mass spectrometry NanoSIMS ; neurodegeneration; peptides;

    Abstract : Alzheimer’s disease (AD) is the most common form of dementia. AD has been linked to the aggregation of amyloid beta (Aβ) peptides into extracellular deposits, Aβ plaques. These are also found in cognitively unimpaired amyloid-positive (CU-AP) individuals, but these Aβ plaques are primarily diffuse in structure. READ MORE