Search for dissertations about: "N-glycosylation"

Showing result 1 - 5 of 19 swedish dissertations containing the word N-glycosylation.

  2. 1. Development and application of alternative methods for profiling proteins N-glycosylation

    Author : Alessandro Quaranta; Leopold Ilag; Niclas Karlsson; Stockholms universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; N-glycosylation; Glycomics; Glycosylation Biomarkers; Intact Glycoproteins; Glycoform Quantification; Mass Spectrometry; Ion Mobility Spectrometry; MALDI-MS; Paper Spray Ionization; Microfluidics; Magnetic Beads; Immunoaffinity Purification; Nanobodies; Analytical Chemistry; analytisk kemi;

    Abstract : Glycosylation is a post-translational modification (PTM) that exerts profound structural and functional effects on the modified protein. Glycan synthesis and conjugation to proteins are regulated by a myriad of factors, both genetic and environmental, and are also influenced by external stressors. READ MORE

  4. 2. Aspects of N-glycosylation in human IgE

    Author : Johanna Da Luz; Karolinska Institutet; Karolinska Institutet; []
    Keywords : ;

    Abstract : The medical interest of allergy in industrialised societies has generated a much greater knowledge about the pathophysiology of IgE-mediated responses than about its normal physiological role. Because of the central role of IgE in allergic inflammation, neutralisation of effector functions and inhibition of IgE-synthesis are attractive means of therapy. READ MORE

  5. 3. Heterologous expression and characterization of xyloglucan endotransglycosylases from Populus tremula x tremuloides

    Author : Åsa Kallas; KTH; []
    Keywords : xyloglucan endotransglycosylase; transglycosylation; hybrid aspen; cell expansion; heterologous expression; Pichia pastoris; N-glycosylation; X-ray crystallography;

    Abstract : Xyloglucan endotransglycosylases (XETs) are enzymes involvedin the reorganization of the primary cell wall. This thesisdescribes several aspects of XET16A from hybrid aspen (Populustremula x tremuloides). The gene was isolated from the hybridaspen cDNA library and the protein was expressed heterologouslyin the methylotrophic yeast Pichia pastoris. READ MORE

  6. 4. Tools for the development of intensified perfusion processes for mammalian cell culture

    Author : Hubert Schwarz; Véronique Chotteau; Mark Smales; KTH; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Recombinant protein biomanufacturing; Perfusion process; Integrated continuous bioprocessing; Monoclonal antibody; Erythropoietin; Chinese Hamster Ovary cells; Human Embryonic Kidney cells; Perfusion medium optimization; Cell specific perfusion rate; Raman spectroscopy; N-glycosylation; Microbioreactors; Design of Experiments; Biotechnology; Bioteknologi;

    Abstract : Recombinant protein therapeutics have become an indispensable part of modern medicine to treat a wide variety of diseases. Their manufacture is mostly based on mammalian cells with fed-batch production bioreactors and downstream batch purification unit operations. READ MORE

  7. 5. Targeting and function of CAH1 : Characterization of a novel protein pathway to the plant cell chloroplast

    Author : Stefan Burén; Göran Samuelsson; Muriel Bardor; Umeå universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Arabidopsis; chloroplast; endomembrane system; CAH1; protein targeting; N-glycosylation; Molecular biology; Molekylärbiologi; molekylärbiologi; Molecular Biology;

    Abstract : The chloroplast is the organelle within a plant cell where photosynthesis takes place. This organelle originates from a cyanobacterium that was engulfed by a eukaryotic cell. READ MORE