Search for dissertations about: "NADH kinase"

Found 5 swedish dissertations containing the words NADH kinase.

  2. 1. Bioelectrochemical Applications of Reactions Catalyzed by Immobilized Enzymes

    Author : Xiao-Jing Tang; Centrum för analys och syntes; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Acetate Kinase.; NAD NADH recycling; Enzyme Electrodes; Enzyme reactors; Enzyme Immobilization; Electrochemical detection; Flow Injection Analysis; Analytical chemistry; Analytisk kemi;

    Abstract : The sensitivity of biosensors can be increased substantially by incorporation in a substrate recycling scheme. Based on the amplification of NAD+/NADH by the recycling reaction catalyzed by glycerol dehydrogenase (GDH) and diaphorase, NAD+/NADH can be determined amperometrically with around 1000 times higher sensitivity than for the substrate sensing mode. READ MORE

  4. 2. Microbial production of 3-hydroxypropionic acid and poly(3-hydroxypropionate): Investigation of Lactobacillus reuteri propanediol utilization pathway enzymes

    Author : Ramin Sabet Azad; Bioteknik; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Platform chemicals; biopolymers; 3-hydroxypropionic acid; 3-hydroxypropionaldehyde; glycerol; poly 3-hydroxypropionate ; polyhydroxyalkanoates; Escherichia coli; Lactobacillus reuteri; propanediol utilization pathway; propionaldehyde dehydrogenase; biotransformation; cofactor regeneration; NADH oxidase;

    Abstract : Concerns regarding environmental issues such as greenhouse gas emissions and climate change have led to a shift within the research community and chemical and energy industry sectors for finding sustainable routes for producing fuels and chemicals from renewable resources, thereby minimizing our dependence on petroleum. The C3-chemical 3-hydroxypropionic acid has been identifed as a top candidate for the biobased chemical industry. READ MORE

  5. 3. Development of bioluminescent reagents for kinetic analysis and some applications in clinical chemistry

    Author : Inger Styrélius; Stockholms universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES;

    Abstract : Firefly luciferase and a bacterial luciferase system have been used to develop analytical methods suitable for use in clinical analysis.The influence of the level of NADH:FMN oxidoreductase on the kinetics of light emission in the bacterial luciferase system was studied. READ MORE

  6. 4. Nitrogen and Redox Metabolism in Saccharomyces cerevisiae

    Author : Eva Albers; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Saccharomyces cerevisiae; glutamate; protein expression; anaerobic physiology; nitrogen metabolism; redox; serine;

    Abstract : Anaerobic conversion of glucose to ethanol by the yeast Saccharomyces cerevisiae has been studied. Glycerol is formed as a by-product in order to maintain the overall redox balance. READ MORE

  7. 5. In vitro studies of the diabetic condition using cultured fibroblasts with focus on wound healing

    Author : Karin M Hehenberger; Karolinska Institutet; Karolinska Institutet; []
    Keywords : diabetes; fibroblasts; wound healing; proliferation; protein kinaseC; lactate; growth factors; resistance.;

    Abstract : This thesis focuses on the diabetic condition at the cellular level, and how this may lead to late complications. Defect wound healing in diabetic patients is poorly understood, but impaired granulation is observed clinically. We have therefore decided to study an in vitro system using cultured fibroblasts. READ MORE