Search for dissertations about: "Oligonucleotide"

Showing result 1 - 5 of 151 swedish dissertations containing the word Oligonucleotide.

  2. 1. Rational modifications of cell-penetrating peptides for drug delivery : Applications in tumor targeting and oligonucleotide delivery

    Author : Maarja Mäe; Ülo Langel; Edvard Smith; Stockholms universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Cell-penetrating peptide; oligonucleotide; drug delivery; tumor targeting; Neurochemistry; Neurokemi; Neurochemistry and Molecular Neurobiology; neurokemi med molekylär neurobiologi;

    Abstract : High molecular weight biomolecules are becoming important in the development of new therapeutics. However, their size and nature creates a major limitation for their application – poor penetration through biological membranes. READ MORE

  4. 2. Cell-penetrating peptides in protein mimicry and oligonucleotide delivery : Applications and mechanisms

    Author : Henrik Johansson; Ülo Langel; Michael Gait; Stockholms universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; peptide; oligonucleotide; cell-penetrating peptide; PNA; splicing; Neurochemistry; Neurokemi; Neurochemistry and Molecular Neurobiology; neurokemi med molekylär neurobiologi;

    Abstract : The plasma membrane functions as a barrier, restricting entry of hydrophilic pharmaceutical agents. Cell-penetrating peptides (CPPs) are capable of transporting bioactive cargos into the cell and have consequently been extensively investigated for their mechanism of entry and capability to deliver various cargos spanning from peptides to plasmids. READ MORE

  5. 3. Novel Methods for Synthesis of High Quality Oligonucleotides

    Author : Andrey Semenyuk; Marek Kwiatkowski; Ulf Landegren; Joachim W. Engels; Uppsala universitet; []
    Keywords : Organic chemistry; oligonucleotide; solid-phase synthesis; RNA synthesis; phosphoramidite; abasic sites; depurination; 2 -O-DTM; protecting group; cartridge; conjugate; dithiomethyl linker; base-stable linker; oligonucleotide purification; nucleoside; Organisk kemi;

    Abstract : The first part of the work describes a procedure of oligonucleotide purification using a reversed-phase cartridge. The developed method employs a very efficient yet mild oligonucleotide detritylation on the cartridge support allowing fast purification of oligonucleotides regardless of their 5´-modification. READ MORE

  6. 4. Specificity of antisense oligonucleotide derivatives and cellular delivery by cell-penetrating peptides

    Author : Peter Guterstam; Ülo Langel; Matthew Wood; Stockholms universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; cell-penetrating peptide; splice-switching oligonucleotide; oligonucleotide derivative; Neurochemistry; Neurokemi; Neurochemistry and Molecular Neurobiology; neurokemi med molekylär neurobiologi;

    Abstract : Atypical gene expression has a major influence on the disease profile of several severe human disorders. Oligonucleotide (ON) based therapeutics has opened an avenue for compensating deviant protein expression by acting on biologically important nucleic acids, mainly RNAs. READ MORE

  7. 5. Complexes of cell-penetrating peptides with oligonucleotides : Structure, binding and translocation in lipid membranes

    Author : Luis Daniel Ferreira Vasconcelos; Ülo Langel; William C. Wimley; Stockholms universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Cell-penetrating peptide; oligonucleotide; transfection; non-covalent complexes; membrane interaction; neurokemi med molekylär neurobiologi; Neurochemistry with Molecular Neurobiology;

    Abstract : The fundamental element of life known to man is the gene. The information contained in genes regulates all cellular functions, in health and disease. The ability to selectively alter genes or their transcript intermediates with designed molecular tools, as synthetic oligonucleotides, represents a paradigm shift in human medicine. READ MORE