Search for dissertations about: "Patch clamp and potassium channels"

Showing result 1 - 5 of 9 swedish dissertations containing the words Patch clamp and potassium channels.

2. 1. Studies of individual pancreatic -cells : Electrophysiological analysis of rhythmic behaviour and development of new techniques

   Author : Staffan Dryselius; Uppsala universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Cell biology; Diabetes; pancreatic beta-cells; insulin release; signal transduction; cytoplasmic calcium; oscillations; patch clamp; ATP-regulated potassium channels; voltage-dependent calcium channels; metabolism; calcium ion; strontium ion; manganese ion; wavelets; de-noising; pattern recognition; superfusion; solution changes; microscopic labelling; Cellbiologi; Cell biology; Cellbiologi; medicinsk cellbiologi; Medical Cell Biology;

   Abstract : The insulin concentration in blood varies periodically, which is believed to prevent down-regulation of the hormone receptors. Loss of the regular insulin oscillations is considered to be an early sign of developing diabetes. READ MORE

4. 2. Molecular aspects on voltage-sensor movement

   Author : Amir Broomand; Fredrik Elinder; Guiscard Seebohm; Linköpings universitet; []
   Keywords : Potassium channels; voltage-gated; chemistry; metabolism; physiology; patch-clamp techniques; oocytes; MEDICINE; MEDICIN;

   Abstract : Voltage-gated ion channels are fundamental for electrical signaling in living cells. They are composed of four subunits, each holding six transmembrane helices, S1-S6. Each subunit contains a voltage-sensor domain, S1-S4, and a pore domain, S5-S6. READ MORE

5. 3. Role of potassium channels in regulating neuronal activity

   Author : Göran Klement; Karolinska Institutet; Karolinska Institutet; []
   Keywords : Potassium channels; threshold dynamics; trafficking; hERG; SMOCs; excitability;

   Abstract : The firing behaviour of excitable cells is fundamental for the information processing in multicellular organisms, varying from single spikes to different forms of repetitive firing. Of the many regulators, voltage gated potassium channels play a major role. In this thesis some aspects of the potassium channel regulation of firing are explored. READ MORE

6. 4. Short- and long-term effects of long-chain free fatty acids on pancreatic α- and β-cell function

   Author : Charlotta Olofsson; Institutionen för experimentell medicinsk vetenskap; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; diabetologi; sekretion; Endokrinologi; diabetology; secreting systems; Endocrinology; type-2 diabetes.; intracellular free Ca2 ; exocytosis; Free fatty acids; pancreatic α- and β-cells;

   Abstract : We have investigated the in vitro effects of long-chain free fatty acids (FFAs) on mouse pancreatic α- and β-cells by a combination of electrophysiological recordings, intracellular Ca2+ ([Ca2+]i) microfluorimetry and hormone release measurements. Acute addition of the saturated FFA palmitate to intact islets doubled insulin as well as glucagon secretion in the presence of 1 or 15 mM glucose and the FFA remained capable of enhancing release of both hormones in islets depolarised with high K+ in the presence of the KATP channel opener diazoxide, indicating that alterations of ATP sensitive potassium (KATP) channel activity is not part of the effect. READ MORE

7. 5. Calcium signaling in astroglial cells. Noradrenergic effects and regulation

   Author : Håkan Muyderman; Göteborgs universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Astrocytes; Neurons; Noradrenaline; Glutamate; Fluorometry; Fura-2; Fluo-3; PBFI; Digital imaging; Voltage-patch-clamp; Whole-cell currents; Calcium; Potassium; Intercellular communication; Glutamate release; Neuron-glial interactions.;

   Abstract : Astroglial cells are the most common cell type in the central nervous system and express a wide array of ion channels and neurotransmitter receptors. The cells organize into multicellular networks and exhibit a calcium-based excitability in which receptor-mediated calcium elevations propagate as intercellular waves over considerable distances. READ MORE