Search for dissertations about: "Patch-Clamp Techniques"

Showing result 1 - 5 of 12 swedish dissertations containing the words Patch-Clamp Techniques.

2. 1. Studies of individual pancreatic -cells : Electrophysiological analysis of rhythmic behaviour and development of new techniques

   Author : Staffan Dryselius; Uppsala universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Cell biology; Diabetes; pancreatic beta-cells; insulin release; signal transduction; cytoplasmic calcium; oscillations; patch clamp; ATP-regulated potassium channels; voltage-dependent calcium channels; metabolism; calcium ion; strontium ion; manganese ion; wavelets; de-noising; pattern recognition; superfusion; solution changes; microscopic labelling; Cellbiologi; Cell biology; Cellbiologi; medicinsk cellbiologi; Medical Cell Biology;

   Abstract : The insulin concentration in blood varies periodically, which is believed to prevent down-regulation of the hormone receptors. Loss of the regular insulin oscillations is considered to be an early sign of developing diabetes. READ MORE

4. 2. Synaptic Integration of Hippocampal Neurons Generated in the Adult Brain: Influence of a Pathological Environment

   Author : Katherine Jakubs; Lund Neurologi; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; neuropsykologi; neurofysiologi; Neurologi; synaptic plasticity; GABA; glutamate; GFP; Neurology; neuropsychology; neurophysiology; hippocampus; dentate gyrus; neurogenesis; LPS; inflammation; epilepsy; SE; patch-clamp;

   Abstract : The adult brain constantly produces new neurons from endogenous neural progenitor cells located in at least two regions, the subgranular zone (SGZ) in dentate gyrus of the hippocampus, and the subventricular zone (SVZ). Major efforts are underway to discover the functional significance of new neurons in the adult brain. READ MORE

5. 3. Molecular aspects on voltage-sensor movement

   Author : Amir Broomand; Fredrik Elinder; Guiscard Seebohm; Linköpings universitet; []
   Keywords : Potassium channels; voltage-gated; chemistry; metabolism; physiology; patch-clamp techniques; oocytes; MEDICINE; MEDICIN;

   Abstract : Voltage-gated ion channels are fundamental for electrical signaling in living cells. They are composed of four subunits, each holding six transmembrane helices, S1-S6. Each subunit contains a voltage-sensor domain, S1-S4, and a pore domain, S5-S6. READ MORE

6. 4. Microfluidics based techniques for electrophysiological studies of cells

   Author : Aikeremu Ahemaiti; Chalmers tekniska högskola; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES;

   Abstract : This thesis work investigates the application of microfluidics to perform electrophysiological studies on cells, including investigations of the effect of cholesterol on the dynamic ion permeability of TRPV1 ion channels, and the application of a microfluidic device, the multifunctional pipette, in electrophysiological studies on brain slices. In the first part of this thesis, Chinese hamster ovary (CHO) cells overexpressing the TRPV1 ion channel were used in a dynamic ion permeability study, where the activation properties of the TRPV1 ion channel were investigated using the patch clamp technique after depletion of membrane cholesterol. READ MORE

7. 5. Transducer properties of a mechanoreceptor : an electrophysiological and pharmacological study of the crayfish stretch receptor

   Author : Jiahui Lin; Karolinska Institutet; Karolinska Institutet; []
   Keywords : stretch receptor; Na channels; K channels; local anesthetics; calmodulin antagonist; patch clamp; voltage clamp;

   Abstract : The abdominal stretch receptor organ of the crayfish, an analog to the human muscle spindle, contains one slowly adapting (SA) and one rapidly adapting (RA) receptor neuron. The general aims of this project were to elucidate, in the SA and RA neurons, the participation of voltagegated Na+ and K+ channels in overall transducer process and adaptation, and to study possible blocking agents specific to mechanosensitive (MS) channels. READ MORE