Search for dissertations about: "RNAseq"

Showing result 1 - 5 of 21 swedish dissertations containing the word RNAseq.

  2. 1. Validation of antibodies for tissue based immunoassays

    Author : Sandra Andersson; Anna Asplund; Fredrik Pontén; Kenneth Wester; Mike Taussig; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Antibody validation; Conjugation; Estrogen receptor-beta; IHC; ISH; Lymphohematopoietic tissues; Proteomic; RNAseq; TMA; Transcriptomic; Patologi; Pathology;

    Abstract : In situ protein detection in human tissues using antibodies reveals the cellular protein localization, and affinity-based proteomic studies can help to discover proteins involved in the development of diseases. However, antibodies often suffer from cross-reactivity, and the lack of positive and negative tissue controls for uncharacterized proteins complicates the mapping of the proteome. READ MORE

  4. 2. Neutrophil and endothelial cell-mediated inflammation in abdominal sepsis

    Author : Zhiyi Ding; Kirurgi; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; sepsis; lung; neutrophil; endothelial cell; neutrophil extracellular traps; inflammation; capillary; post capillary venules; cytoskeleton; RNAseq;

    Abstract : Sepsis is defined as a life-threatening condition caused by a dysregulated host response to infection. Neutrophils are themost abundant innate immune cells of the body and play a key role in septic pathogenesis. READ MORE

  5. 3. The interplay between Giardia intestinalis and host intestinal epithelial cells : A tale of immune activation and suppression

    Author : Jana Grüttner; Staffan Svärd; Mikael E. Sellin; Steven Singer; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Giardia; diarrhea; RNAseq; proteomics; small intestinal epithelial cells; enteroids; organoids; protozoan; differentiation; immune response; host-parasite interaction; swimming; Biologi med inriktning mot mikrobiologi; Biology with specialization in Microbiology;

    Abstract : Giardia intestinalis is a non-invasive, protozoan parasite causing the diarrheal disease giardiasis in a variety of mammals, including humans. The parasite has two main life cycle stages; disease-causing trophozoites and infectious cysts. Trophozoites colonize the host’s upper small intestine by attaching to intestinal epithelial cells (IECs). G. READ MORE

  6. 4. Function and Evolution of Small Regulatory RNAs and their Associated Proteins : A Journey from Genome to Proteome

    Author : Bart Edelbroek; Fredrik Söderbom; Jonathan R. Chubb; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; microRNAs; non-coding RNAs; Evolution; Whole Genome Sequencing; Transcriptomics; Proteomics; small RNAseq; Amoebozoa; Multicellular development; Social amoebae; Comparative genomics; Genome annotation; Biologi med inriktning mot mikrobiologi; Biology with specialization in Microbiology;

    Abstract : Organisms throughout the tree of life have evolved distinct ways to regulate gene expression. Some of these processes involve non-coding RNAs (ncRNAs), which are not translated but functional nonetheless. These ncRNAs are of utmost importance, with dysregulation of some causing severe developmental effects or even being lethal. READ MORE

  7. 5. Resolving the Cellular Heterogeneity of Human Hematopoietic Stem and Progenitor Cells. The influence of cellular heterogeneity on ontogeny, ageing and leukemia

    Author : Mikael Sommarin; Avdelningen för molekylär hematologi; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Hematopoiesis; Human; Single-Cell; HSC; Leukemia; LSC; HSPC; sc-RNAseq; sc-ATAC-seq;

    Abstract : .... READ MORE