Search for dissertations about: "RNase III"

Showing result 1 - 5 of 22 swedish dissertations containing the words RNase III.

  2. 1. Post-transcriptional regulation by RNases in Streptococcus pyogenes

    Author : Anne-Laure Lécrivain; Emmanuelle Charpentier; Bernt Eric Uhlin; Tracy Nissan; Ciarán Condon; Umeå universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Post-transcriptional regulation; mRNA decay; RNA sequencing; RNase Y; RNase III; YhaM; PNPase; RNase R; RNase J1; Streptococcus pyogenes.; molekylärbiologi; Molecular Biology;

    Abstract : Ribonucleases (RNases) are proteins that adjust cellular RNA levels by processing RNA transcripts, leading to their stabilization or degradation. RNases are grouped based on their ability to cleave the transcript internally (endoRNases) or degrade the transcript starting from the ends (exoRNases). READ MORE

  4. 2. Functional characterization of the small antisense RNA MicA in Escherichia coli

    Author : Klas Ifeanyi Udekwu; Gerhart Wagner; Tony Romeo; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; small RNA; antisense; outer membrane; Hfq-dependence; RNase III; LuxS; Quorum sensing; Microbiology; Mikrobiologi; mikrobiologi; Microbiology;

    Abstract : The Escherichia coli small RNA (sRNA) MicA was identified recently in a genomewide search for sRNAs. It is encoded between the genes gshA and luxS in E. coli and its close relatives. The function of sRNAs in bacteria is generally believed to be in maintenance of homeostasis via stress-induced modulation of gene expression. READ MORE

  5. 3. Conformationally Constrained Oligonucleotides for RNA Targeting

    Author : Qing Li; Jyoti Chattopadhyaya; Li-He Zhang; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; conformationally constrained nucleoside; antisense oligonucleotide; RNA affinity; nuclease stability; RNase H; RNA degradation; Chemistry with specialization in Bioorganic Chemistry; Kemi med inriktning mot bioorganisk kemi;

    Abstract : A short oligonucleotide sequence as in a single-stranded antisense oligo nucleotides (AON) or in double-stranded small interfering RNAs (siRNA) can modulate the gene expression by targeting against the cellular mRNA, which can be potentially exploited for therapeutic purposes in the treatment of different diseases. In order to improve the efficacy of oligonucleotide-based drugs, the problem of target affinity, nuclease stability and delivery needs to be addressed. READ MORE

  6. 4. Targeting RNA by the Antisense Approach and a Close Look at RNA Cleavage Reaction

    Author : Jharna Barman; Lars Baltzer; Roger Strömberg; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Organic chemistry; mRNA targeting; antisense oligonucleotides; target affinity; RNase H; Michaelis-Menten kinetics; exo-nuclease stability; NMR; pH titration; alkaline hydrolysis; Organisk kemi;

    Abstract : This thesis summarizes the results of studies on two aspects of nucleic acids. Chemically modified antisense oligonucleotides (AONs) have been evaluated with regards to their suitability for mRNA targeting in an antisense approach (Paper I – III). READ MORE

  7. 5. Structure, function and metabolic stability of antisense RNAs

    Author : Fredrik Söderbom; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Microbiology; Mikrobiologi; Microbiology; Mikrobiologi; mikrobiologi; Microbiology;

    Abstract : Antisense RNAs usually are small, highly structured, unstable RNAs that control a varietyof different biological processes. They exert their effect by binding to complementarytarget RNAs and thereby inhibit their functions. READ MORE