Search for dissertations about: "Triplet State imaging"

Showing result 1 - 5 of 8 swedish dissertations containing the words Triplet State imaging.

  2. 1. Transient State Fluorescence Microscopy - method development and biological applications : Exploiting the dark states of fluorophores to measure oxygen concentrations, redox state, Förster resonance energy transfer and membrane viscosity

    Author : Thiemo Spielmann; Jerker Widengren; Karsten König; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Transient States imaging TRAST ; Triplet State imaging; fluorescence microscopy; modulated excitation; triplet state; radical state; trans-cis isomerisation;

    Abstract : Due to their long lifetime, triplet, redox and other transient states of fluorophores are highly sensitive to the micro-environment. Imaging their spatial distribution in biological samples can therefore help answer interesting questions about the metabolism, molecular interactions and dynamics in living cells. READ MORE

  4. 2. Monitoring Proton Exchange and Triplet States with Fluorescence

    Author : Tor Sandén; Jerker Widengren; Vogel Horst; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; fluorescence correlation spectroscopy; proton transfer; cytochrome c oxidase; transient state imaging; modulated excitation; Optics; Optik; Biochemistry; Biokemi;

    Abstract : Fluorescent molecules commonly shift to transient dark states, induced bylight or triggered by chemical reactions. The transient dark states can beused as probes of the local environment surrounding the fluorescent molecules,and are therefore attractive for use in biomolecular applications. READ MORE

  5. 3. Photo-induced dark states influorescence spectroscopy – investigations & applications

    Author : Andriy Chmyrov; Jerker Widengren; Johan Hofkens; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; fluorescence correlation spectroscopy; triplet state; isomerisation; photobleaching; quenching; diffusion; total internal reflection; interface; Spectroscopy; Spektroskopi;

    Abstract : This thesis focuses on investigations of transient dark states of fluorescentmolecules using spectroscopic techniques. The main purpose is to show andconvince the reader that transient dark states are not always a nuisance, butalso represent an additional source of information. READ MORE

  6. 4. Synthesis and development of compounds for nonlinear absorption of light

    Author : Tomas Kindahl; Bertil Eliasson; Sascha Ott; Umeå universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Platinum acetylide; nonlinear absorption; optical power limiting; oxazole; quantum chemistry calculation; quantum chemical calculation; triplet absorption; organisk kemi; Organic Chemistry; fysikalisk kemi; Physical Chemistry; Quantum Chemistry; kvantkemi;

    Abstract : High-intensity light — for instance that from a laser — can be destructive, not only to the human eye, but also to equipment such as imaging sensors and optical communication devices. Therefore, effective protection against such light is desirable. READ MORE

  7. 5. Temporal Modulation in Fluorescence Spectroscopy and Imaging for Biological Applications

    Author : Gustav Persson; Jerker Widengren; Jörg Enderlein; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; fluorescence; spectroscopy; microscopy; modulated excitation; intensity modulation; fluorescence correlation spectroscopy; transient states; molecular kinetics; Physics; Fysik;

    Abstract : This thesis explores the benefits of intensity modulation for the purpose of extending the range of applications of fluorescence spectroscopy and imaging in cellular and molecular biology and medicine. Long-lived transient states of fluorescent molecules can, because of their long lifetimes, be used to detect subtle changes in the microenvironment of the molecule. READ MORE