Search for dissertations about: "UHPLC"

Showing result 1 - 5 of 15 swedish dissertations containing the word UHPLC.

  2. 1. Fundamental and Regulatory Aspects of UHPLC in Pharmaceutical Analysis

    Author : Dennis Åsberg; Torgny Fornstedt; Jörgen Samuelsson; Gert Desmet; Karlstads universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Liquid chromatography; UHPLC; Pharmaceutical analysis; Adsorption isotherm; Design of experiments; Quality control; Kemi; Chemistry;

    Abstract : Ultra-high performance liquid chromatography (UHPLC) provides a considerable increase in throughput compared to HPLC and a reduced solvent consumption. The implementation of UHPLC in pharmaceutical analysis, e.g. READ MORE

  4. 2. Mass Spectrometry of Biologically Active Small Molecules : Focusing on polyphenols, alkaloids and amino acids

    Author : Zdeněk Spáčil; Leopold L. Ilag; Frantisek Turecek; Stockholms universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; LC-MS; LDI; Mass spectrometry; HPLC; UHPLC; polyphenols; phenolic acids; BMAA; Separation methods; Separationsmetoder; Analytical chemistry; Analytisk kemi; Analytical Chemistry; analytisk kemi;

    Abstract : The foci of this dissertation are on advanced liquid chromatography (LC) separation and mass spectrometry (MS) techniques for the analysis of small bioactive molecules. In addition to discussing general aspects of such techniques the results from analyses of polyphenols (PPs), alkaloids and amino acids published in five appended studies are presented and discussed. READ MORE

  5. 3. Analysis of Metabolites in Complex Biological Samples Using LC/MS and Multivariate Data Analysis : Metabolic Fingerprinting and Detection of Biomarkers

    Author : Helena Idborg; Sven Jacobsson; Per-Olof Edlund; Jonas Bergquist; Stockholms universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Metabolomics; Metabonomics; Metabolic Fingerprinting; Drug Metabolism; Biomarkers; Human Plasma; Rat Urine; LC MS; ZIC-HILIC; UPLC; UHPLC; ToF; Curve Resolution; Peak Detection; Pattern Recognition; Classification; PCA; PLS; PARAFAC; N-PLS; Data Fusion; Hierarchical Modeling; Batch modeling; Data Concatenation; Breast Cancer; Phospholipidosis; Analytical chemistry; Analytisk kemi; Analytical Chemistry; analytisk kemi;

    Abstract : To facilitate early diagnosis of diseases and elucidation of the processes involved in their development and progression, various specific compounds or ‘biomarkers’ are often monitored. The first step is to decide which compounds to analyze. READ MORE

  6. 4. Adsorption Studies with Liquid Chromatography : Experimental Preparations for Thorough Determination of Adsorption Data

    Author : Lena Edström; Jonas Bergquist; Lars Hagel; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Liquid chromatography; HPLC; UHPLC; Reversed phase; Preparative chromatography; Adsorption isotherm; Injection profile; Sample pH; pH stable conditions; Peak deformation; Band distortion; Overloaded band; Chiral preparative chromatography; Analytical Chemistry; Analytisk kemi;

    Abstract : Analytical chemistry is a field with a vast variety of applications. A robust companion in the field is liquid chromatography, the method used in this thesis, which is an established workhorse and a versatile tool in many different disciplines. READ MORE

  7. 5. Selectivity in NMR and LC-MS Metabolomics : The Importance of Sample Preparation and Separation, and how to Measure Selectivity in LC-MS Metabolomics

    Author : Albert Elmsjö; Curt Pettersson; Torbjörn Arvidsson; Thomas Moritz; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Metabolomics; NMR; LC-MS; HILIC; UHPLC; Q-ToF; selectivity; co-feature ratio; method evaluation; data evaluation; Analytical Pharmaceutical Chemistry; Analytisk farmaceutisk kemi;

    Abstract : Until now, most metabolomics protocols have been optimized towards high sample throughput and high metabolite coverage, parameters considered to be highly important for identifying influenced biological pathways and to generate as many potential biomarkers as possible. From an analytical point of view this can be troubling, as neither sample throughput nor the number of signals relates to actual quality of the detected signals/metabolites. READ MORE