Search for dissertations about: "affinity reagents"
Showing result 1 - 5 of 36 swedish dissertations containing the words affinity reagents.
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1. Protein microarrays for validation of affinity binders
Abstract : Is specificity an important issue regarding affinity reagents? What about the validation of affinity reagents today, is it good enough? This depends on the application and the producer of the reagent. Validation should be the most important marketing argument that can be found. READ MORE
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2. Investigations of chemical and enzymatic functionalization of affinity proteins
Abstract : AbstractAffinity proteins are important reagents in research, diagnostics and therapeutic settings. The focus of this thesis has been on investigating different chemical and enzymatic strategies for engineering of affinity proteins to generate affinity reagents with improved or changed functionality. READ MORE
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3. Affibody ligands in immunotechnology applications
Abstract : This thesis describes the development and use ofnon-immunoglobulin affinity proteins denoted affibodies asalternatives to antibodies in different immunotechnologyapplications. A 58 aa IgG Fc binding three-helix bundle domainZ, derived from staphylococcal protein A has been used asframework for library constructions, in which the face of themolecule involved in the native binding activity has beenengineered by combinatorial protein engineering. READ MORE
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4. Molecular Recognition in Antibody Engineering. Studies on recombinant and phage displayed antibodies
Abstract : In vitro generation of antibody fragments of desired specificity and affinity plays an important role since it permits the generation of reagents which may be valuable for diagnostic and therapeutic applications. Furthermore, the kinetic parameters for an antibody-antigen interaction, rather than the affinity, has shown to correlate with biological functions like virus neutralization. READ MORE
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5. Bioimaging for analysis of protein expression in cells and tissues using affinity reagents
Abstract : The detection and analysis of biomolecules, such as proteins, are of great interest since these molecules are fundamental for life and our health. Due to the complexity of biological processes, there is a great advantage of studying proteins in their natural context, for example by using bioimaging. READ MORE