Search for dissertations about: "aminotransferase"

Showing result 1 - 5 of 34 swedish dissertations containing the word aminotransferase.

2. 1. Fighting Tuberculosis – : Structural Studies of Three Mycobacterial Proteins

   Author : Alina Castell; Torsten Unge; Manfred Weiss; Uppsala universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Mycobacterium tuberculosis; Rv0216; Rv0130; Mycobacterium smegmatis; branched chain aminotransferase; X-ray crystallography; fatty acid metabolism; Structural biology; Strukturbiologi;

   Abstract : This thesis presents the cloning, purification, crystallization, and structural studies of two unknown proteins from Mycobacterium tuberculosis, and of an aminotransferase from Mycobacterium smegmatis. Structural knowledge of these proteins is of highest interest for structure-based drug design, which is one of the approaches that can be used in order to fight tuberculosis (TB). READ MORE

4. 2. Enzymatic mechanisms in biotin synthesis : vitamin B6 catalysis and phosphoryl transfer

   Author : Jenny Sandmark; Karolinska Institutet; Karolinska Institutet; []
   Keywords : X-ray crystallography; biotin biosynthesis; pyridoxal-5 -phosphate; aminotransferase; aluminium fluoride; S-adenosyl-L-methionine; 7; 8-diaminopelargonic acid synthase; dethiobiotin synthetase; biotin; phosphoryl transfer; amiclenomycin.;

   Abstract : With the large number of pathogenic bacteria that have developed antibiotic resistance it is increasingly important to find new targets for antibiotics. Our interest in the biotin biosynthesis pathway originates from this drug design perspective. All organisms require biotin, but it is only synthesised in bacteria, in plants and in some fungi. READ MORE

5. 3. Counter-current distribution of interacting molecules : simulation of distribution behaviour and application to protein-protein interactions

   Author : Lars Backman; Umeå universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES;

   Abstract : Associations of biological macromolecules with other macromolecules, with larger assemblies of macromolecules and with themselves are widely encountered phenomena. In principle, these interactions can be studied with any method able to differentiate between free molecules and complexes formed. READ MORE

6. 4. Cellular mechanisms involved in bone resorption

   Author : Ulf Lerner; Umeå universitet; []
   Keywords : Bone resorption. cyclic AMP; parathyroid hormone; prostaglandins; la OH D3; lysosomal enzyme release; osteoclast; periodontitis; rheumatoid arthritis;

   Abstract : The effects of parathyroid hormone (PTH), prostaglandins (PGE1, PGE2, PGF2a), cAMP, cAMP-analogues, phosphodiesterase (PDE) inhibitors and la (OH) D3 on bone resorption and associated cellular process have been studied in a bone organ culture system using half- calvaria from 6-7 day-old mice. Bone resorption was assessed by determining the release of calcium (Ca2+), inorganic phosphate (Pi) and 45Ca from the calvarial bones to the culture media. READ MORE

7. 5. Mitochondrial differentiation during the early development of the amphibian embryo

   Author : Lennart Nelson; Barbara Cannon; Umeå universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Amphibia; Xenopus laevis; Ambystoma mexicanum; mitochondria; differentiation; enzymes; carriers; oxidation; development; Zoophysiology; zoofysiologi;

   Abstract : Mitochondria from Xenopus laevis and Ambystoma mexica- num embryos between fertilization and the beginning of feeding were studied: the former with respect to metabolic behaviour, enzyme pattern and carrier activity, and the latter with respect to morphological parameters.The metabolic behaviour of mitochondria was studied by assessing the rates of oxygen uptake in presence of various substrates. READ MORE