Search for dissertations about: "biofysik"

Showing result 16 - 20 of 682 swedish dissertations containing the word biofysik.

  2. 16. From Static Structures to Free Energy Landscapes: Characterizing Conformational Transitions in Biological Macromolecules

    Author : Cathrine Bergh; Erik Lindahl; Gerhard Hummer; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Protein conformational transitions; molecular dynamics simulations; statistical mechanics; ion channels; Proteinkonformationsövergångar; molekyldynamiksimuleringar; statistisk mekanik; jonkanaler;

    Abstract : To be alive means to temporarily counteract the fundamental dispersive driving force described by the second law of thermodynamics, eventually leading all systems to decay and disorder. In cells, this task is partly carried out by proteins - small specialized molecular machines that utilize free energy to maintain the proper functioning of the cell. READ MORE

  4. 17. 3D super-resolution microscopy of living cells using reversibly switchable fluorophores

    Author : Andreas Bodén; Ilaria Testa; Francisco Balzarotti; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; RESOLFT; 3D imaging; super-resolution; microscopy; reversibly switchable fluorescent proteins; Biological Physics; Biologisk fysik;

    Abstract : Traditional optical microscopy techniques are limited in spatial resolution due to the wave nature of light. This means that neighboring objects separated by a distance smaller than about 200 nm cannot be distinguished. READ MORE

  5. 18. Automated super-resolution microscopy for high-throughput imaging

    Author : Xavier Casas Moreno; Ilaria Testa; Florian Jug; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; RESOLFT; throughput; microscopy automation; nanoscopy; Biologisk fysik; Biological Physics;

    Abstract : Fluorescence microscopes enable the visualization of biological samples with high contrast by labeling specific structures with fluorescent molecules. However, the spatial resolution of widely used microscopy techniques, such as widefield and confocal microscopy, is limited by the size of a focused spot of light, which roughly corresponds to half the wavelength of the illumination. READ MORE

  6. 19. Investigation of Neuronal Protein Trafficking at the Molecular Scale

    Author : Martina Damenti; Ilaria Testa; Clive Bramham; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Endoplasmic Reticulum; Mitochondria; Arc Activity-regulated cytoskeleton-associated protein ; oligomerization; pRESOLFT; STED; event-triggered FCS; DNA-PAINT; STARSS; molecular dynamics simulations; Biological and Biomedical Physics; Biologisk och biomedicinsk fysik;

    Abstract : Neurons are polarized cells that encode information in the nervous system viaelectrochemical connections named synapses. The tuning of synapticconnections is enabled by a plastic protein trafficking system which operates atthe nanoscale to finely tweak the neuronal ultrastructure. READ MORE

  7. 20. Advancing tissue clearing and expansion methods for high-resolution volumetric imaging of biological samples

    Author : Steven Edwards; Hjalmar Brismar; Etsuo Susaki; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biologisk fysik; Biological Physics;

    Abstract : The development of advanced light microscopes, capable of imaging samples at ever-higher spatial resolution and increasing speeds is an ongoing endeavour. The sample itself is an integral part of the microscope and, unlike the intricately positioned and highly polished lenses, it is an optically unpredictable component. READ MORE