Search for dissertations about: "bispecific"

Showing result 1 - 5 of 26 swedish dissertations containing the word bispecific.

  2. 1. An albumin-binding domain as a scaffold for bispecific affinity proteins

    Author : Johan Nilvebrant; Sophia Hober; Andrew Bradbury; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; albumin-binding domain; bispecific; albumin; affinity protein; phage display; staphylococcal display; orthogonal affinity purification; TNF-alpha; ErbB2; ErbB3; SRA - Molecular Bioscience; SRA - Molekylär biovetenskap;

    Abstract : Protein engineering and in vitro selection systems are powerful methods to generate binding proteins. In nature, antibodies are the primary affinity proteins and their usefulness has led to a widespread use both in basic and applied research. READ MORE

  4. 2. Brain distribution of a bispecific antibody targeting Aβ

    Author : Gustavsson Tobias; Dag Sehlin; Stina Syvänen; Paul O'Callaghan; Danica Stanimirovic; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Alzheimer s disease; amyloid-beta; blood-brain barrier; single-photon emission computed tomography SPECT ; bispecific antibody; immunotherapy; Molekylär medicin; Molecular Medicine; Neuroscience; Neurovetenskap;

    Abstract : Alzheimer’s disease (AD) is characterised by aberrant protein aggregation in the brain with subsequent synaptic loss, neuroinflammation, and brain atrophy that ultimately clinically manifests as cognitive impairment. Histopathological findings in AD are extracellular plaques of the protein amyloid-beta (Aβ), Aβ in blood vessels (CAA), and intracellular neurofibrillary tangles (NFT) of hyperphosphorylated tau. READ MORE

  5. 3. Strategies to improve and balance the expression levels of recombinant proteins in mammalian cell lines

    Author : Mona Moradi Barzadd; Johan Rockberg; Alan Dickson; KTH; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; CHO; HEK293; Aggregation; Bispecific; Cancer assay; Cell line engineering; sulfatase; biologics; transcriptomic; Biotechnology; Bioteknologi;

    Abstract : Proteins are the building blocks of all living organisms enabling us to function and survive. There are more than 100,000 different proteins in the human body performing a variety of vital tasks. READ MORE

  6. 4. Engineering strategies for ABD-derived affinity proteins for therapeutic and diagnostic applications

    Author : Mikael Åstrand; Sophia Hober; John Löfblom; Larry Gold; KTH; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; ABD; ADAPT; Protein G; ERBB2; ERBB3; Directed evolution; phage display; staphylococcal display; bispecific; Bioteknologi; Biotechnology;

    Abstract : Small stable protein domains are attractive scaffolds for engineering affinity proteins due to their high tolerance to mutagenesis without loosing structural integrity. The albuminbinding domain is a 5 kDa three-helix bundle derived from the bacterial receptor Protein G with low-nanomolar affinity to albumin. READ MORE

  7. 5. EGFR- and HER2-Binding Affibody Molecules : Cellular studies of monomeric, dimeric and bispecific ligands

    Author : Lina Ekerljung; Lars Gedda; Johan Lennartsson; Jörgen Carlsson; Birgitta Sundström; Uppsala universitet; []
    Keywords : EGFR; HER2; Affibody molecule; cell signaling; receptor dimerization; Biomedical Radiation Science; Biomedicinsk strålningsvetenskap;

    Abstract : Abnormal expression and signaling of the ErbB receptors is associated with the development and progression of several forms of cancer. In this thesis, new ErbB-targeting affibody molecules are evaluated regarding their cellular effects in vitro. READ MORE