Search for dissertations about: "carbohydrate binding module"

Showing result 1 - 5 of 17 swedish dissertations containing the words carbohydrate binding module.

  2. 1. Novel binding specificities engineered into the scaffold of a carbohydrate binding module

    Author : Lavinia Cicortas Gunnarsson; Institutionen för immunteknologi; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Bioteknik; Biotechnology; carbohydrate binding module; binding specificity; selection; combinatorial library; scaffold; protein engineering;

    Abstract : The growing field of biotechnology is in constant need of proteins that can function as recognition tools for separational, analytical and therapeutic purposes. Different molecular engineering approaches are applied on natural proteins in order to create variants with desired properties. READ MORE

  4. 2. Functional studies and engineering of family 1 carbohydrate-binding modules

    Author : Janne Lehtiö; KTH; []
    Keywords : Cellulose-binding; carbohydrate-binding modules; phage display; bacterial surface display; combinatorial protein library; protein engineering; Trichoderma reesei; Staphylococcus carnosus;

    Abstract : The family 1 cellulose-binding modules (CBM1) form a groupof small, stable carbohydrate-binding proteins. These modulesare essential for fungal cellulosedegradation. This thesisdescribes both functional studies of the CBM1s as well asprotein engineering of the modules for several objectives. READ MORE

  5. 3. Heterologous expression, characterization and applications of carbohydrate active enzymes and binding modules

    Author : Åsa Kallas; Tuula T. Teeri; Edward A. Bayer; KTH; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; LANTBRUKSVETENSKAPER; AGRICULTURAL SCIENCES; Populus; xyloglucan endotransglycosylase; carbohydrate binding modules; endo- 1; 4 -b-xylanase; Escherichia coli; Pichia pastoris; N-glycosylation; fiber analysis; Bioengineering; Bioteknik; Wood fibre and forest products; Träfiber- och virkeslära;

    Abstract : Wood and wood products are of great economical and environmental importance, both in Sweden and globally. Biotechnology can be used both for achieving raw material of improved quality and for industrial processes such as biobleaching. READ MORE

  6. 4. On the engineering of proteins: methods and applications for carbohydrate-active enzymes

    Author : Fredrika Gullfot; Harry Brumer; Antoni Planas; KTH; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; enzyme engineering; rational design; directed evolution; DNA shuffling; glycosynthase; xyloglucan; xyloglucan endo-transglycosylase; retaining glycoside hydrolase; xyloglucanase; carbohydrate binding module; polysaccharide synthesis; Bioengineering; Bioteknik;

    Abstract : This thesis presents the application of different protein engineering methods on enzymes and non-catalytic proteins that act upon xyloglucans. Xyloglucans are polysaccharides found as storage polymers in seeds and tubers, and as cross-linking glucans in the cell wall of plants. READ MORE

  7. 5. Engineering protein-carbohydrate interactions - lessons from natural and evolved carbohydrate binding modules

    Author : Laura von Schantz; Institutionen för immunteknologi; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; carbohydrate binding modules protein engineering affinity affinity maturation phage display specificity crystallography cellulose derivatives;

    Abstract : Protein-carbohydrate interactions are of central role in all living organism. In the studies presented in this thesis several strategies for engineering such interactions have been investigated. In the first study the binding affinity of a carbohydrate binding module (CBM) was improved a 100-fold through affinity maturation. READ MORE