Search for dissertations about: "circle-to-circle amplification"

Found 5 swedish dissertations containing the words circle-to-circle amplification.

2. 1. Detection and Sequencing of Amplified Single Molecules

   Author : Rongqin Ke; Mats Nilsson; Ulf Landegren; Radoje Drmanac; Uppsala universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; padlock probes; proximity ligation assay; rolling circle amplification; circle-to-circle amplification; single molecule detection; amplified single molecules; sequencing; in situ; single cell; Molecular Biotechnology; Molekylär bioteknik;

   Abstract : Improved analytical methods provide new opportunities for both biological research and medical applications. This thesis describes several novel molecular techniques for nucleic acid and protein analysis based on detection or sequencing of amplified single molecules (ASMs). READ MORE

4. 2. Visual and magnetic detection of antimicrobial resistance genes using nanoparticles and isothermal amplification

   Author : Darío Sánchez Martín; Teresa Zardán Gómez de la Torre; Maria Strömme; Mahiar Hamedi; Uppsala universitet; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; DNA amplification; isothermal amplification; visual detection; nanoparticles; VAM-NDA; rolling circle amplification; phi29 DNA polymerase; padlock probes.; Teknisk fysik med inriktning mot nanoteknologi och funktionella material; Engineering Science with specialization in Nanotechnology and Functional Materials;

   Abstract : Antibiotic resistance is becoming an increasing concern, necessitating new ways to diagnose and treat it rapidly and accurately. One proposed solution is the use of nucleic acid amplification tests, capable of detecting resistance in just a few hours, as opposed to the several days that traditional testing methods may require. READ MORE

5. 3. Single Molecule Detection : Microfluidic Automation and Digital Quantification

   Author : Malte Kühnemund; Mats Nilsson; Ulf Landegren; Sten Linnarsson; Uppsala universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; single molecule; digital; rolling circle amplification; magnetic particle; padlock probe; microfluidics; resistive pulse sensing; lab on chip; mobile phone microscopy; enrichment; sequencing;

   Abstract : Much of recent progress in medical research and diagnostics has been enabled through the advances in molecular analysis technologies, which now permit the detection and analysis of single molecules with high sensitivity and specificity. Assay sensitivity is fundamentally limited by the efficiency of the detection method used for read-out. READ MORE

6. 4. Selector Technology : For Multiplex DNA Analysis

   Author : Fredrik Dahl; Mats Nilsson; Ulf Landegren; Ivo Glynne Gut; Uppsala universitet; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Genetic engineering; DNA technology; Genteknik; Genetic engineering including functional genomics; Genteknik inkl. funktionsgenomik;

   Abstract : A majority of methods for identifying sequences in the human genome involve target sequence amplification through PCR. This work presents novel methods for amplifying circularized DNA and presents solutions for some major limitations of PCR. READ MORE

7. 5. Cell and organoid models to develop new antivirals and diagnostic methods for emerging viruses

   Author : Aleksandra Pettke; Karolinska Institutet; Karolinska Institutet; []
   Keywords : ;

   Abstract : The ongoing global pandemic of severe acute respiratory syndrome coronavirus 2 (SARSCoV-2) is only the latest reminder of the urgent need for new antiviral strategies and diagnostic tools to improve preparedness for emergence of new pathogenic RNA-viruses. A key obstacle for the development of antivirals and diagnostic tools has been the lack of good models to study RNA viruses. READ MORE