Search for dissertations about: "cloning vector"

Showing result 1 - 5 of 8 swedish dissertations containing the words cloning vector.

  2. 1. Protein production and purification in structural genomics

    Author : Martin Hammarström; Per-Åke Nygren; Torleif Härd; Helena Berglund; Herman van Tilbeurgh; KTH; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Recombinational cloning; Escherichia coli; gene expression; fusion proteins; solubility; circular dichroism; nuclear magnetic resonance; Structural biochemistry; Strukturbiokemi;

    Abstract : The number of gene products available for structural and functional study is increasing at an unprecedented rate as a result of the successful whole genome sequencing projects. Systematic structure determination of proteins on a genomic scale, called structural genomics, can significantly contribute to the field of protein science and to functional annotation of newly identified genes. READ MORE

  4. 2. Expression of recombinant neutralizing anti-HIV-1 antibodies in bacteria and eukaryotic cells

    Author : Fayezeh Yari; Karolinska Institutet; Karolinska Institutet; []
    Keywords : Phage display; vector; monoclonal antibody; antigen; eukaryotic; prokaryotic; transfection; selection; transient expression; stable cell line; neutralizing antibody; expression; HIVA-1; gp41; gp120;

    Abstract : The main purpose of this study was to investigate and optimize techniques for eukaryotic expression of antibody molecules (IgG, Fab and scFV-ê formats), derived from an immune phage displayed Fab library and reactive against HIV-1 glycoproteins gp120 and gp41. Light and heavy-chain (Fd) DNA from a number of Fab clones were transferred from a prokaryotic phagemid vectors to mammalian expression vectors. READ MORE

  5. 3. Specific genetic modifications in the CNS - Cell specific expression and gene specific regulation

    Author : Erika Elgstrand; CNS Genterapi; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Parkinson’s disease Gene therapy Cell specific GAD67 Rat Striatum Substantia nigra pars reticulata;

    Abstract : Gene therapy is a promising therapeutic tool for many diseases of the CNS. Lentiviral vectors are particularly attractive since this vector type can transduce both dividing and non-dividing cells, have a relatively large cloning capacity, can sustain long-term transgene expression and have low immunogenicity. READ MORE

  6. 4. Genetic vaccination against acute viral disease

    Author : Marina N Fleeton; Karolinska Institutet; Karolinska Institutet; []
    Keywords : Semliki Forest virus; Alphavirus. Influenza A virus; DNA vaccines. Influenza vaccine. Respiratory Syncytial Viruses; Louping Ill. Flavivirus Infections;

    Abstract : This thesis describes the development of recombinant vaccines based on the Semliki Forest virus (SFV) expression system. Immunisation of mice with recombinant virus particles, a layered DNA/RNA plasmid vector, and recombinant self-replicating RNA were carried out and the protective effect of these recombinant vaccines against viral challenge were examined. READ MORE

  7. 5. Bark beetles facilitate the establishment of wood decay fungi

    Author : Ylva Strid; Sveriges lantbruksuniversitet; Sveriges lantbruksuniversitet; []
    Keywords : LANTBRUKSVETENSKAPER; AGRICULTURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES;

    Abstract : Forests in the northern hemisphere are largely dominated by conifers and provide a key habitat for a multitude of species. Wood decay fungi, i.e. basidiomycetes, are important for nutrient cycling. READ MORE