Search for dissertations about: "conditioned medium"

Showing result 1 - 5 of 45 swedish dissertations containing the words conditioned medium.

2. 1. Regulation of productivity in Trichoplusia ni and Spodoptera frugiperda Sf9 serum-free cultures

   Author : Karin Calles; Lena Häggström; Gunnel Dalhammar; KTH; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Biotechnology; Insect cells; BEVS; Spodoptera frugiperda; Sf9; Trichoplusia ni; BTI-Tn-5B1-4; specific productivity; conditioned medium; conditioned medium factors; cell cycle; synchronization; G2 M; Bioteknik; Bioengineering; Bioteknik;

   Abstract : The aim of this work has been to characterize the effects of conditioned medium (CM) on insect cell productivity and physiology in order to get a better understanding about the mechanisms that regulate productivity in serum-free media. Two cell lines have been investigated, Spodoptera frugiperda (Sf9) and Trichoplusia ni (T. ni, BTI-Tn-5B1-4). READ MORE

4. 2. Physiological effects of conditioned medium and passage number on Spodoptera frugiperda Sf9 serum free cultures

   Author : Ingrid Svensson; Lena Häggström; Mats Martinelle; KTH; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Biotechnology; Spodoptera frugiperda Sf9; insect cell cultures; conditioned medium; cell cycle phase dynamics; proteolytic activity; antibacterial activity; recombinant protein production; Bioteknik; Bioengineering; Bioteknik;

   Abstract : The aim of this study was to better understand the role of conditioned medium (CM) in Spodoptera frugiperda Sf9 insect cell proliferation and recombinant protein production using the baculovirus expression system. CM was found to stimulate cell proliferation. READ MORE

5. 3. Development of a protein-free fed-batch process for NS0 cells: studies on regulation of proliferation

   Author : Erika Spens; Lena Häggström; Jean-Louis Goergen; KTH; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NS0 myeloma cells; protein-free medium; fed-batch; conditioned medium; autocrine growth factors; abortive proliferation; protease activity; aprotinin; gp130; Bioengineering; Bioteknik;

   Abstract : The overall objective of this study was to investigate how NS0 cell proliferation is regulated in protein-free media. The hypothesis was that during the adaptation to growth factor-free media, animal cell lines start to produce their own autocrine growth factors to support proliferation, and after some time in a culture the effects of these factors are lost which results in cessation of proliferation. READ MORE

6. 4. Impact of autocrine factors on physiology and productivity in Trichoplusia ni serum-free cultures

   Author : Ulrika Eriksson; Lena Häggström; Per Berglund; KTH; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Biotechnology; Trichoplusia ni; High Five; insect cells; BEVS; serum-free medium; yeast extract; conditioned medium; autocrine regulation of proliferation; growth factors; cell cycle; metalloproteinase; specific productivity; Bioteknik; Bioengineering; Bioteknik;

   Abstract : The aim of this study was to increase the understanding of the mechanisms regulating cell proliferation and recombinant protein production in serum-free cultures of Trichoplusia ni (T. ni) insect cells.Conditioned medium (CM) was shown to contain both stimulatory and inhibitory factors (CM factors) influencing cell growth. READ MORE

7. 5. Optimizing stem cells for reconstructive surgery

   Author : Anne Therese Lauvrud; Paul J. Kingham; Rebecca Wiberg; Peyman Kelk; Maria Brohlin; Susanna Kauhanen; Umeå universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Adipose tissue-derived stem cells; liposuction; oncological safety; xenofree growth medium; Human Anatomy; anatomi;

   Abstract : Fat grafting has become an established method in plastic surgery for treating soft tissue defects. The results for survival of the fat being transplanted is unpredictable and supplementation of the graft with the Stromal Vascular Fraction (SVF) or cultures Adipose tissue-derived stem cells (ASCs) can enhance graft viability. READ MORE