Search for dissertations about: "crosslinking mass spectrometry"

Showing result 1 - 5 of 8 swedish dissertations containing the words crosslinking mass spectrometry.

  2. 1. Exploring small heat shock protein chaperones by crosslinking mass spectrometry

    Author : Wietske Lambert; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; cross-linking; mass spectrometry; protein-protein interactions; Hsp21; BS3; DTSSP; MALDI-TOF TOF; client protein; substrate; aggregation; unfolded protein; chaperone; Small heat shock protein; crosslinking;

    Abstract : Together with other molecular chaperones, small heat shock proteins are key components of the protein quality control system, which is comprised of several hundred proteins and acts to maintain proteome homeostasis in the cell. Small heat shock proteins bind unfolding proteins at an early stage, to prevent these from further unfolding and aggregating. READ MORE

  4. 2. Combined structural methods to characterize an oligomeric small heat shock protein Hsp21

    Author : Gudrun Rutsdottir; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Small heat shock proteins; chaperones; crosslinking mass spectrometry; Stable isotope labeling; quantification; small angle X-ray scattering; cryo-electron microscopy; combined structural methods; plant stress;

    Abstract : Small heat shock proteins (sHsps) are found in all organisms and evolved early in the history of life, prior to the divergence of the three domains of life (Archaea, Bacteria, and Eukarya). Their malfunction is connected to several different diseases in humans, such as Alzheimer’s and Parkinson’s disease. READ MORE

  5. 3. The Molecular Chaperone DNAJB6 - A Suppressor of Disease Related Amyloid Fibril Formation

    Author : Cecilia Månsson; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; DNAJ; HSP40; molecular chaperones; amyloid fibrils; Alzheimer’s disease; Huntington’s Disease; crosslinking mass spectrometry; chemical kinetics; aggregation; fibril formation;

    Abstract : Several neurodegenerative diseases are caused by peptides or proteins forming amyloid fibrils such as the Aβ peptide involved in Alzheimer’s disease and the huntingtin exon-1 with a prolonged polyglutamine (polyQ) stretch involved in Huntington’s disease. The ability to form amyloid fibrils is an intrinsic feature in all proteins. READ MORE

  6. 4. Molecular Imaging of Diabetic Kidney Tissue and Binding Studies of Proinsulin C-peptide

    Author : Lina Lindfors; Jan Kihlberg; Ingela Lanekoff; Fredrik Almqvist; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Diabetic kidney disease; Proinsulin C-peptide; GPR146; Mass spectrometry imaging; nano-DESI; Kemi; Chemistry;

    Abstract : Diabetic kidney disease is a serious complication of diabetes with a complex and incompletely understood pathology. In this work, the molecular changes in diabetic rat kidneys at a very early disease stage were studied using nanospray desorption electrospray ionisation mass spectrometry imaging. READ MORE

  7. 5. The Role of FCGBP in Mucus - Structure, Processing and Function

    Author : Erik Ehrencrona; Göteborgs universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Mucus; FCGBP; IgG; IBD; COPD; Mucins; Disulphide; GDPH;

    Abstract : In this thesis, a bottom-up approach was used to study the IgG Fc-binding protein (FCGBP), which next to the MUC2 mucin is the second main component of secreted mucus in small intestine and colon. FCGBP is also found in airways during inflammatory conditions with static mucus. READ MORE