Search for dissertations about: "cryogenic electron microscopy"

Showing result 1 - 5 of 38 swedish dissertations containing the words cryogenic electron microscopy.

  2. 1. Transmission Electron Microscopy of Graphene and Hydrated Biomaterial Nanostructures : Novel Techniques and Analysis

    Author : Sultan Akhtar; Klaus Leifer; Hilborn Jöns; Stefano Rubino; Marco Cantoni; Uppsala universitet; []
    Keywords : Graphene flakes; magnetic beads DNA coils; hydrated biomaterials; transmission electron microscopy; focused ion beam scanning electron microscopy; bright-field dark-field imaging; high resolution imaging; electron diffraction and cryogenic temperatures; Materialvetenskap; Materials Science;

    Abstract : Transmission Electron Microscopy (TEM) on light element materials and soft matters is problematic due to electron irradiation damage and low contrast. In this doctoral thesis techniques were developed to address some of those issues and successfully characterize these materials at high resolution. READ MORE

  4. 2. Light’EM up! : structural characterization of light-driven membrane protein complexes by cryogenic electron microscopy

    Author : André Tomás Graça; Wolfgang P. Schröder; Johannes Messinger; Karina Persson; Werner Kühlbrandt; Umeå universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; 3D reconstruction; Arabidopsis thaliana; assembly; chlorophyll; cryo-EM; cyanobacteria; Deinococus radiodurans; electron; land plants; membrane protein complexes; Photosystem II; proton; single-particle analysis; S-Layer Deinoxanthin Binding Complex SDBC ; water.; Biochemistry; biokemi; biologisk kemi; biological chemistry;

    Abstract : Photosynthesis is probably the most important process for allowing life to develop into the diverse forms we see today. In this process, solar radiation is used to convert CO2 into biomass. From this process, we obtain oxygen to breathe, sources of food (plant biomass), and the potential for clean and sustainable energy. READ MORE

  5. 3. Fast and reliable alignment and classification of biological macromolecules in electron microscopy images

    Author : Björn Forsberg; Erik Lindahl; Sriram Subramaniam; Stockholms universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; cryo-EM; electron microscopy; GPU; parallel processing; protein structure; Biochemistry towards Bioinformatics; biokemi med inriktning mot bioinformatik;

    Abstract : In the last century, immense progress has been made to charter and understand a wide range of biological phenomena. The origin of genetic inheritance was determined, showing that DNA holds genes that determine the architecture of proteins, utilized by the cell for most functions. READ MORE

  6. 4. Association and Microstructure in Self-Assembling Catanionic Systems

    Author : Eduardo Marques; Fysikalisk kemi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; PFG NMR self-diffusion; cryogenic-transmission electron microscopy; catanionic systems; gels; bile salts; polyelectrolytes; liquid crystals; vesicles; Surfactants; phase diagrams; light microscopy; Theoretical chemistry; quantum chemistry; Teoretisk kemi; kvantkemi;

    Abstract : This thesis addresses the phase behavior and phase structure in mixed surfactant-surfactant and surfactant-polymer systems, where the co-solutes have opposite charges (catanionic systems). The initial goal has been to investigate self-assembly aspects in mixtures of single-/double-chained surfactants and bile salt/double-chained surfactants, with the main focus on vesicle assembly in dilute regions. READ MORE

  7. 5. Structural studies of drug targets and a drug metabolizing enzyme

    Author : Daniela Cederfelt; U. Helena Danielson; Doreen Dobritzsch; Inari Kursula; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Biophysics; Protein structure; X-ray crystallography; Cryogenic electron microscopy; Enzymology; Drug discovery; Pyrimidine degradation; Biokemi; Biochemistry;

    Abstract : The work presented in this thesis describes how structural information about a protein can be acquired, and how it can be used to answer scientific questions about proteins’ function, their dynamic behaviour and their interactions with other proteins or ligands.The catalytic function of the pyrimidine-degrading, drug metabolizing enzyme β-ureidopropionase (βUP) is dependent on the shift between oligomeric states. READ MORE