Search for dissertations about: "dUTP"

Showing result 1 - 5 of 9 swedish dissertations containing the word dUTP.

  2. 1. Nucleotide Binding to Trimeric dUTPase: Studies on Recombinant Enzymes using Kinetic and Spectroscopic Methods

    Author : Johan Nord; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; C-terminus; dUTPase; EIAV; Escherichia coli; flexibility; Homo sapiens; kinetics; NMR; nucleotide metabolism; rate constants of lig-and binding; production of recombinant enzyme; dUTP; specificity; Biochemistry; Metabolism; Biokemi;

    Abstract : Recombinant dUTPase from the bacterium Escherichia coli, the retrovirus equine infectious anemia virus (EIAV) and Homo sapiens have been made available for physico-chemical characterisation. The protocols developed have also proven to be suitable for production of enzymes modified by isotope labelling or other methods. READ MORE

  4. 2. On Monomeric and Trimeric dUTPases. Recombinant Expression, Purification, Conformational Properties and Catalytic Characteristics

    Author : Rebecca Persson; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Metabolism; dUTP; dUTPase; nucleotide metabolism; retrovirus; crystallography; herpesvirus; Biokemi; Biochemistry;

    Abstract : Nucleotide metabolism and replication of nucleic acids are processes of fundamental importance. A crucial enzyme in nucleotide metabolism is dUTPase (E.C. 3. READ MORE

  5. 3. Photoluminescence-based characterization of bioengineered nanovesicles and erbium emitters

    Author : Maryam Sanaee; Katia Gallo; Jerker Widengren; Martin Hof; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Bioengineered nanovesicles; Coincident fluorescent burst analysis; Drug loading; Erbium emitters; Lithium niobate; Photoluminescence.; Bioingenjörskonstruerade nanovesiklar; Analys av sammanfallande fluorescerande blossteknik; Läkemedelslastning; Erbium-emitterare; Lithium niobate; Fotoluminiscens.; Biologisk fysik; Biological Physics; Optics and Photonics; Optik och fotonik;

    Abstract : In recent decades, photoluminescence properties of single molecules and ions have opened new possibilities for studies on smaller size scales, below the light-diffraction limit. In this thesis, the advantages of such single photon emitters were harnessed and studied mainly in the field of biophysics, but also, in investigations within solid state photonics. READ MORE

  6. 4. Homotrimeric dUTPases : Principles of Catalysis and Inhibitor Design

    Author : Lorena Gonzalez Palmén; Jan Kvassman; Dolores Gonzalez-Pacanowska; Högskolan i Kalmar; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; dUTPases; kinetics; ground state destabilization; inhibitors; Biochemistry; Biokemi; Natural Science; Naturvetenskap;

    Abstract : The ubiquitous enzyme dUTPase hydrolyzes dUTP into dUMP and pyrophosphate, preventing DNA fragmentation and cell death due to accumulation of dUTP. Inhibitors of dUTPase could serve as drugs in the treatment of cancers and infectious diseases. This thesis presents five studies. READ MORE

  7. 5. Viral dUTPases, Recombinant Expression, Purification and Characterization

    Author : Anna-Carin Bergman; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Proteins; nucleocapsid protein; retrovirus; herpesvirus; dUTPase; nucleotide metabolism; enzymology; Proteiner; enzymologi;

    Abstract : The enzyme deoxyuridine 5’-triphosphate nucleotidohydrolase (dUTPase, EC 3.6.1.23) catalyzes the hydrolysis of dUTP to dUMP and pyrophosphate. READ MORE