Search for dissertations about: "detection tag"

Showing result 1 - 5 of 42 swedish dissertations containing the words detection tag.

  1. 1. Strategies for Miniaturized Biomarker Detection

    Author : Belinda Adler; Avdelningen för Biomedicinsk teknik; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; biomarker; miniturization; lab-on-a-chip; proteomics; PSA; microarray; mass spectrometry; MALDI MS; SPE; sample preparation; immunoaffinity; antibody; aptamer; mass tag; ISET.;

    Abstract : The aim of this thesis is development and application of different miniaturized strategies for detection of biomarkers. The biomarker PSA (prostate specific antigen), which is prostate cancer specific, has been the main focus of the thesis. READ MORE

  2. 2. Improved detection and performance of surface expression from the AIDA-I autotransporter

    Author : Johan Jarmander; Gen Larsson; Ulrika Rova; Ines Ezcurra; KTH; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; surface expression; autotransport; AIDA-I; Escherichia coli; proteolysis; detection tag;

    Abstract : Surface expression of recombinant proteins has attracted a lot of attention due to its potential in applications such as enzyme production, vaccine delivery and bioremediation. Autotransporters have been used for surface expression of a variety of proteins, but the expression systems reported in literature have typically been inflexible and incapable of detecting proteolysis, thereby limiting surface expression yield. READ MORE

  3. 3. Metal Affinity Peptides in Biotechnological Applications As tools in protein purification, protein stabilisation, metal quantification and metal tolerance of bacteria and plants

    Author : Malin Mejàre; Tillämpad biokemi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; cadmium; metal quantification; fusion tag; metal affinity tag; phage display; peptide library; metal tolerance; metal resistance; metal accumulation; bioremediation; transgenic plant; tobacco; potato; protein purification; immobilised metal affinity chromatography; metal affinity precipitation; metal detection; protein stabilisation; Bioteknik; Biotechnology; Biokemi; Metabolism; Biochemistry; metal ion;

    Abstract : Metal affinity tags have been fused to proteins and demonstrated to function as tools in protein purification, protein stabilisation, quantitative metal analysis and metal tolerance of bacteria and plants. In biotechnological applications proteins frequently have to function in nonnatural environments with harsh conditions which impose requirements for high stability on the proteins. READ MORE

  4. 4. Biomolecular Analysis by Dual-Tag Microarrays and Single Molecule Amplification

    Author : Olle Ericsson; Ulf Landegren; Mats Nilsson; Edwin Southern; Uppsala universitet; []
    Keywords : Molecular medicine; Microarray; proximity ligation; Padlock probe; Rolling circle amplification; Splicing; single molecule; Molekylärmedicin;

    Abstract : Padlock probes and proximity ligation are two powerful molecular tools for detection of nucleic acids and proteins, respectively. Both methods result in the formation of DNA reporter molecules upon recognition of specific target molecules. READ MORE

  5. 5. Impulse Radio UWB for the Internet-of-Things : A Study on UHF/UWB Hybrid Solution

    Author : Zhuo Zou; Hannu Tenhunen; Tor Sverre Lande; KTH; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Ultra-Wideband; impulse radio; IR-UWB; RFID; asymmetric links; UHF UWB hybrid; wireless sensing; energy detection; low power; radio receiver; positioning; Internet-of-Things;

    Abstract : This dissertation investigates Ultra-Wideband (UWB) techniques for the next generation Radio Frequency Identification (RFID) towards the Internet-of-Things (IoT). In particular, an ultra-high frequency (UHF) wireless-powered UWB radio (UHF/UWB hybrid) with asymmetric links is explored from system architecture to circuit implementation. READ MORE