Search for dissertations about: "enzyme immunoassay"

Showing result 1 - 5 of 39 swedish dissertations containing the words enzyme immunoassay.

  2. 1. Development and Application of Enzyme- and Antibody-based Assays with Emphasis on Selectivity, Sensitivity and Stability

    Author : Catalin Nistor; Centrum för analys och syntes; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; stability; sensitivity; selectivity; cross-reactivity; sample matrix effects; environmental pollutants; enzyme biosensor; immunoassay; Analytical chemistry; Analytisk kemi;

    Abstract : This thesis presents the development, optimisation, and various applications of enzyme-based biosensors and immunoassays, mainly with relevance to environmental analysis. the thesis discusses problems and possible solutions associated with selectivity, sensitivity and robustness of such bioassays with emphasis on various immunoasssay configurations for determination of surfactants, phenols, or drugs, and amperometric biosensors based on enzymes (e. READ MORE

  4. 2. Enzyme Immunoassay in Combination with Liquid Chromatography for Sensitive and Selective Determination of Drugs in Biosamples

    Author : Ulf Lövgren; Centrum för analys och syntes; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Analytical chemistry; amplification; alkaline phosphatase; enzyme immunoassay; liquid chromatography; flow-injection; micellar; Analytisk kemi;

    Abstract : Two different sensitive microtitre-plate based enzyme immunoassays were developed and evaluated for determination of the model drug compound, budesonide, in blood plasma. In the first approach, high sensitivity was obtained by using amperometric detection in a flow injection system for determination of the enzyme product p-aminophenol. READ MORE

  5. 3. Studies of immune responses to cell surface proteins of Helicobacter pylori and Borrelia burgdorferi by enzyme immunoassay and immunoblotting

    Author : Ingrid Nilsson; Avdelningen för medicinsk mikrobiologi; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Mikrobiologi; mycology; virology; B. burgdorferi; H.pylori; cell surface proteins; gel-electrophoresis; enzyme immunoassay; Western immunoblotting; bacteriology; Microbiology; bakteriologi; virologi; mykologi;

    Abstract : Helicobacter pylori and Borrelia burgdorferi s.l. can cause chronic infections by evading the immune system. These two human pathogens express a number of immunogenic cell surface proteins. READ MORE

  6. 4. Rapid sample preparation and bioanalytical techniques for efficient screening of organic pollutants in the environment

    Author : Malin Nording; Peter Haglund; Ron Hoogenboom; Umeå universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; accelerated solvent extraction; CAFLUX; food; immunoassay; PAH; PCDD; PCDF; pressurized liquid extraction; soil; CALUX; cell-based bioassay; chemically activated fluorescent gene expression; chemically activated luciferase gene expression; dioxin; ELISA; enzyme-linked immunosorbent assay; feed; Environmental chemistry; Miljökemi;

    Abstract : Large numbers of samples often need to be prepared and analysed in surveys of organic pollutants in the environment, but while the methods commonly used in such surveys can provide abundant detail they are generally costly, time-consuming and require large amounts of resources, so there is a need for simpler techniques. The work underlying this thesis assessed the potential utility of more convenient sample preparation and bioanalytical techniques for rapidly screening various environmental matrices that could be useful complements to higher resolution methods. READ MORE

  7. 5. Design and development of electrochemical sensors with application in bioanalysis

    Author : Kinga Zor; Bioteknik; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; bioanalysis; electrochemistry; sensor; biosensor; miniaturization; real sample; immunoassay; μTAS;

    Abstract : There is a continuous search for reliable, robust, easy to use, portable and cost effective analytical tools with applications in food industry, biomedicine, environmental monitoring and homeland security. The main focus of this thesis is the development of electrochemical (bio)sensors and bioanalysis systems (to be applied in two major fields of interest: food & beverage industry and biomedical research) for monitoring key analytes in complex matrices, such as: meat samples, fermentation broths, and cell culture media. READ MORE