Search for dissertations about: "enzymologi Proteiner enzymology E. coli Immobilised metal ion affinity chromatography Affinity tags"

Found 1 swedish dissertation containing the words enzymologi Proteiner enzymology E. coli Immobilised metal ion affinity chromatography Affinity tags.

2. 1. Metal Binding Tags - Characterisation, Use in Bioseparation and Applications of Green Fluorescent Fusion Proteins

   Author : Florent Bernaudat; Tillämpad biokemi; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Proteiner; enzymologi; enzymology; E. coli; Immobilised metal ion affinity chromatography; Affinity tags; Green fluorescent protein; Surface plasmon resonance; Genetic engineering; Screening; Proteins; Immunoassay; Biotechnology; Bioteknik;

   Abstract : Most of the recombinant proteins produced nowadays are fused to affinity tags, in order to facilitate their purification through affinity chromato-graphy. Out the different tags available, poly-histidine tags are among the most commonly used and can help purification through immobilised metal ion affinity chromatography (IMAC). READ MORE