Search for dissertations about: "fluorescent hybridization probes"

Found 4 swedish dissertations containing the words fluorescent hybridization probes.

2. 1. The Light-up Probe

   Author : Nicke Svanvik; Chalmers tekniska högskola; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; asymmetric cyanine dyes; light-up probes; PNA; real-time PCR; LightUp Probe; fluorescent hybridization probes; homogeneous hybridization assays;

   Abstract : The work presented in this thesis concerns the development of light-up probes and their potential applications in PCR based molecular diagnostics. The light-up probe is a nucleic acid analog (here PNA) oligomer linked to an asymmetric cyanine dye (here thiazole orange) and it combines the excellent hybridization properties of PNA with the large fluorescence enhancement of the dye upon binding to DNA. READ MORE

4. 2. Novel fluorescent tools and techniques for 3D imaging of the cleared brain

   Author : Alex Yu Hin Wong; Karolinska Institutet; Karolinska Institutet; []
   Keywords : ;

   Abstract : Background: To better understand the complexity of the brain and how it becomes impaired under different pathological states, a considerably large number of brains would be needed for imaging to generate highly detailed maps in 3D. Chemical probes can offer a readily scalable labelling method that is robust, easy to use with the quick operation, and feasible for human tissue where genetic viral and toxin tracers are inappropriate. READ MORE

5. 3. Development of DNA-based methods for analysis of protein interactions

   Author : Axel Klaesson; Ola Söderberg; Mats Nilsson; Cristina Al-Khalili Szigyarto; Uppsala universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; In situ proximity ligation assay PLA ; rolling circle amplification RCA ; Hybridization chain reaction HCR ; proxHCR; Oligonucleotide design; Protein-protein interactions; Post-translational modifications.; Farmaceutisk vetenskap; Pharmaceutical Science; Molekylär cellbiologi; Molecular Cellbiology;

   Abstract : In situ proximity ligation assay (PLA) is a method for detection of protein interactions, post-translational modifications (PTMs) and individual proteins that allows information about their localization in a cell or tissue to be extracted. The method is based on oligonucleotide-conjugated antibodies (proximity probes) that upon binding of two epitopes in close proximity give rise to an amplifiable DNA circle. READ MORE

6. 4. Technology development for genome and polymorphism analysis

   Author : Magnus Jobs; Karolinska Institutet; Karolinska Institutet; []
   Keywords : SNP; Genotyping technology; DASH; Array technology; Assay design;

   Abstract : Single nucleotide polymorphism (SNP) is widely expected to provide a multitude of benefits in areas of medical research. There is, however, a growing disparity between the rate of SNP discovery and the development of effective technologies for large scale SNP scoring. READ MORE