Search for dissertations about: "functional assay"

Showing result 1 - 5 of 264 swedish dissertations containing the words functional assay.

  2. 1. Functional studies of calcein acetoxymethyl ester in human tumor cells

    Author : Gunnar Liminga; Uppsala universitet; []
    Keywords : Medical sciences; Calcein AM; Calcein; Functional assay; Drug resistance; Cytotoxicity; MEDICIN OCH VÅRD; MEDICINE; MEDICIN; klinisk farmakologi; Clinical Pharmacology;

    Abstract : A fluorometric assay for functional measurement of P-glycoprotein 170 (Pgp) mediated transport in cell lines, using the acetoxymethyl ester of calcein (calcein/AM) as a probe was established. The myeloma cell line RPMI 8226/S and the two Pgp-expressing doxorubicin resistant subcell lines 8226/dox6 (low) and 8226/dox40 (high) were used. READ MORE

  4. 2. Functional characterization of pro-angiogenic neutrophils

    Author : Jalal Lomei; Mia Phillipson; Gustaf Christoffersson; Johan Kreuger; Lars Olaf Cardell; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; angiogenesis; leukocytes; neutrophils; pro-angiogenic neutrophils; islets transplantation; intravital microscopy; aortic ring assay; phagocytosis; ROS production; hypoxia; vascular permeability; neutrophil recruitment; Medical Cell Biology; Medicinsk cellbiologi;

    Abstract : The vascular system stretches throughout the body to provide oxygen, nutrition, and to remove waste products from cell metabolism. Angiogenesis, formation of new blood vessels by sprouting from pre-existing vessels, is one of the main mechanisms involved in blood vessel formation. READ MORE

  5. 3. Understanding and manipulating primary cell walls in plant cell suspension cultures

    Author : Felicia Leijon; Vincent Bulone; Katja Salomon Johansen; KTH; []
    Keywords : Callose synthase; carbohydrate-binding module; cell wall engineering; cellulose microfibril; cellulose nanocrystal; glycosyltransferase; mass spectrometry; plasmodesmata; Populus; primary cell wall; radiometric assay; spectrophotometric assay; Biotechnology; Bioteknologi;

    Abstract : The cell wall is required for many aspects of plant function and development. It is also an accessible and renewable resource utilized both in unrefined forms and as raw material for further development. Increased knowledge regarding cell wall structure and components will contribute to better utilization of plants and the resources they provide. READ MORE

  6. 4. Cartilage destruction - Release of type IX collagen in joint disease

    Author : Mikael Danfelter; Reumatologi och molekylär skelettbiologi; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; cartilage destruction; matrix metalloproteinase 13; ECM; assay; neoepitope; NC4 domain; Type IX collagen; fragment;

    Abstract : Cartilage is of vital importance for the function of joints. It is characterized by a prominent extracellular matrix (ECM) that is produced and maintained by relatively few cells of one type, the chondrocyte. READ MORE

  7. 5. Cell-penetrating peptides, novel synthetic nucleic acids, and regulation of gene function : Reconnaissance for designing functional conjugates

    Author : Peter Guterstam; Ülo Langel; Stockholms universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Cell-penetrating peptide; nucleic acids; alternative splicing; mismatch discrimination; Neurochemistry; Neurokemi;

    Abstract : Our genome operates by sending instructions, conveyed by mRNA, for the manufacture of proteins from chromosomal DNA in the nucleus of the cell to the protein synthesizing machinery in the cytoplasm. Alternative splicing is a natural process in which a single gene can encode multiple related proteins. READ MORE