Search for dissertations about: "glucose chromatography"

Showing result 1 - 5 of 41 swedish dissertations containing the words glucose chromatography.

  2. 1. Affinity-, Partition- and Permeability Properties of the Human Red Blood Cell Membrane and Biomembrane Models, with Emphasis on the GLUT1 Glucose Transporter

    Author : Christine Lagerquist Hägglund; Per Lundahl; Jørgen Vinten; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Biochemistry; Affinity; Aromatic amino acids; Binding; Biomembrane; Biotin; Chromatography; Cytochalasin B; Dihydrocytochalasin B; Dissociation constant; Drug absorption; Ethanol; Equilibrium; Glucose; GLUT1; Immobilization; Immobilized biomembrane affinity chromatography; Immobilized liposome chromatography; Interaction; Liposome; Membrane protein; Membrane vesicle; Partitioning; Phospholipid bilayer; Proteoliposome; Quantitative; Red blood cell; Specific; Streptavidin; Tyrosine; Tryptophan; Biokemi; Biochemistry; Biokemi;

    Abstract : The human glucose transporter GLUT1 is abundant in red blood cells, the blood-brain barrier and epithelial cells, where it mediates the transport of the energy metabolite, glucose. In the present work some properties of GLUT1, including affinity binding of both substrates and inhibitors, transport rates as well as permeabilities of aromatic amino acids and drug-membrane interactions were analyzed by chromatographic methods. READ MORE

  4. 2. Chromatographic Studies of Solute Interactions with Immobilized Red Blood Cells and Biomembranes

    Author : Ingo Gottschalk; Irving W. Wainer; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Affinity; Binding; Biomembrane; Biotin; Chromatography; Cytochalasin B; Dissociation constant; Drug absorption; Equilibrium; Glucose; GLUT1; Immobilization; Immobilized biomembrane affinity chromatography; Immobilized liposome chromatography; Interaction; Liposome; Membrane protein; Membrane vesicle; Partitioning; Phospholipid bilayer; Proteoliposome; Quantitative; Red blood cell; Solute; Specific; Streptavidin; Wheat germ agglutinin; Biokemi; Biochemistry; Biokemi; Biokemi; Biochemistry;

    Abstract : Specific and non-specific interactions of solutes with immobilized biomembranes were studied using chromatographic methods. Liposomes, proteoliposomes and red blood cell (RBC) membrane vesicles were immobilized by a freeze-thawing procedure, whereas whole RBCs were adsorbed in the gel beds using electrostatic interaction, binding to wheat germ agglutinin (WGA) or the streptavidin-biotin interaction. READ MORE

  5. 3. Development of Flourescence-based Immunosensors for Continous Carbohydrate Monotoring : Applications for Maltose and Glucose

    Author : Henrik Engström; Sten Ohlson; Per Ola Andersson; Christopher Lowe; Högskolan i Kalmar; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Carbohydrate; Diabetes; Immunosensor; ; Glucose; Maltose; Weak affinity chromatograhpy; Kolhydrater; Glukos; Maltose; Affinitetskromatografi; Diabetes; Biochemistry; Biokemi; Bioteknik; Biotechnology;

    Abstract : Weak affinity interaction of monoclonal antibodies and carbohydrate antigens can be detected and quantified by alterations in the antibodies' intrinsic tryptophan fluorescence. These weak/transient binding events have been monitored by total internal reflection fluorescence (TlRF) by facilitating the change in intrinsic tryptophan fluorescence. READ MORE

  6. 4. Quantitation, Purification and Reconstitution of the Red Blood Cell Glucose Transporter GLUT1

    Author : Shusheng Zuo; Bengt Mannervik; Bengt Persson; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Cholesterol; Cytochalasin B; Glucose; GLUT1; Hummel and Dreyer analysis; Immobilization; PEG 5000 -DSPE; Biomembrane; Proteoliposome; Quantitative frontal affinity chromatography; Red blood cell streptavidin-biotin immobilization; Sulfhydryl affinity chromatography; The modified micro-Bradford CaPE assay; MALDI-ToF-MS; Biokemi; Biochemistry; Biokemi;

    Abstract : The human glucose transporter GLUT1 facilitates glucose to be accumulated on the other side of the cell membrane. The functional state of GLUT1 is uncertain due to diversity of the reports. In this thesis, the activity of red blood cell GLUT1 was extensively studied to further characterize this protein. READ MORE

  7. 5. Fast isolation of proteins from bioreactors using affinity chromatography techniques

    Author : Eva Pålsson; Avdelningen för klinisk genetik; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; chromatography; protein purification; analyses; dispersion; chromatography matrices; Biochemistry; Biokemi; Metabolism; expanded bed; metabolism; Biotechnology; Bioteknik;

    Abstract : The purification of proteins from raw materials like fermentation broth and other biological sources usually involves several purification steps and is rather time consuming and expensive. This is a major concern in the production of many important protein products e.g. pharmaceuticals such as insulin and blood coagulation factors. READ MORE