Search for dissertations about: "histidine tag"

Showing result 1 - 5 of 11 swedish dissertations containing the words histidine tag.

2. 1. Molecular regulation of rat histidine decarboxylase

   Author : Christine Dartsch; Institutionen för experimentell medicinsk vetenskap; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Physiology; omeprazole; antiserum; fetal rat liver; COS-7 cells; purification; bacterial expression; protein turnover; ECL cells; gastric mucosa; gastrin; histdine decarboxylase; histamine; Fysiologi; Proteins; enzymology; Proteiner; enzymologi;

   Abstract : Histamine is involved in a variety of physiological processes like gastric acid secretion, inflammation and neurotransmission. The production of histamine is catalysed by the enzyme histidine decarboxylase (HDC), which is a strongly regulated enzyme. READ MORE

4. 2. Recombinant amelogenin - Strategies for purification and protein processing

   Author : Johan Svensson Bonde; Tillämpad biokemi; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Recombinant amelogenin; protein purification; fusion protein; purification tag; enamel; hydroxyapatite; matrix protein; dental regeneration; wound healing; bone formation;

   Abstract : Amelogenin is an extracellular matrix protein involved in formation of enamel during early tooth development. By interacting with the forming enamel crystallites amelogenin affects their growth, so that highly elongated hydroxyapatite crystals are formed. READ MORE

5. 3. A radical view of haemoglobin - protein engineering and characterisation

   Author : Marie Grey; Tillämpad biokemi; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; His-Gln tagging; protein purification; blood substitutes; radical scavenging; superoxide dismutase; E. coli; diglobin; Haemoglobin; protein engineering; aqueous two-phase systems; hydrophobic interaction chromatography;

   Abstract : In recent years shortages of donated blood have become a growing problem for health care systems world-wide. Blood substitutes based on human haemoglobin (Hb) could potentially ameliorate this situation. However, clinical trials have indicated problems due to e.g. READ MORE

6. 4. Metal Binding Tags - Characterisation, Use in Bioseparation and Applications of Green Fluorescent Fusion Proteins

   Author : Florent Bernaudat; Tillämpad biokemi; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Proteiner; enzymologi; enzymology; E. coli; Immobilised metal ion affinity chromatography; Affinity tags; Green fluorescent protein; Surface plasmon resonance; Genetic engineering; Screening; Proteins; Immunoassay; Biotechnology; Bioteknik;

   Abstract : Most of the recombinant proteins produced nowadays are fused to affinity tags, in order to facilitate their purification through affinity chromato-graphy. Out the different tags available, poly-histidine tags are among the most commonly used and can help purification through immobilised metal ion affinity chromatography (IMAC). READ MORE

7. 5. Hydrophobic Interactions in Bioseparation - Applications of Modified Green Fluorescent Protein

   Author : Kristian Becker; Tillämpad biokemi; []
   Keywords : Hydrophobic interaction chromatography; capillary electrochromatography; E. coli; isolation tags; haemoglobin; green fluorescent protein; retention prediction; genetic engineering; accessible surface area;

   Abstract : This thesis describes interactions that are salt-promoted and mainly based on the hydrophobicity of proteins. A series of variants of the green fluorescent protein (GFP) was first constructed by site-directed mutagenesis. Several tags with various properties were then genetically fused to the N-terminus of GFP. READ MORE