Search for dissertations about: "living cells"

Showing result 1 - 5 of 408 swedish dissertations containing the words living cells.

  2. 1. Electrochemical monitoring of living cells

    Author : Christer Spegel; Centrum för analys och syntes; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; PC12; redox; electrochemistry; NAD P H; Single cell; Microchip; S. cerevisiae;

    Abstract : This thesis presents the utilization of electroanalytical methods for analysis of dynamic cellular processes such as exocytosis, intracellular redox reactions and cell surface interactions. Microchips have been designed and manufactured with the purpose of monitoring dopamine exocytosis from single or a small ensemble of PC12 cells. READ MORE

  4. 2. PROBING NAD(P)H AVAILABILITY IN LIVING CELLS USING MINIATURIZED ELECTROCHEMICAL SYSTEMS

    Author : Natalie Kostesha; Teknisk mikrobiologi; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; redox; NAD P H; living cells; S. cerevisiae; mediated amperometry; microelectrode; electrochemistry;

    Abstract : The work presented in this thesis demonstrates the application of electrochemical methods for probing the dynamics of cellular metabolism in prokaryotic and eukaryotic cells. The main part of the work has been done using genetically modified strains of yeast. Employing baker’s yeast, S. READ MORE

  5. 3. lac of Time : Transcription Factor Kinetics in Living Cells

    Author : Petter Hammar; Johan Elf; Antoine van Oijen; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; gene regulation; transcription factor; lac operon; facilitated diffusion; single molecule imaging; Molecular Biotechnology; Molekylär bioteknik;

    Abstract : Gene regulation mediated by transcription factors (TFs) is essential for all organisms. The functionality of TFs can largely be described by the fraction of time they occupy their regulatory binding sites on the chromosome. DNA-binding proteins have been shown to find their targets through facilitated diffusion in vitro. READ MORE

  6. 4. Ultrasonic Handling of Living Cells in Microfluidic Systems

    Author : Jessica Svennebring; Martin Wiklund; Hans Hertz; Martyn Hill; KTH; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Bioengineering; Bioteknik;

    Abstract : Microfluidic chips have become a powerful tool in research where biological cells are processed and/or analyzed. One method for contactless cell manipulation in microfluidic chips that has gained an increasing amount of attention the last decade is ultrasonic standing wave (USW) technology. READ MORE

  7. 5. Imaging Living Cells with an X-ray Laser

    Author : Gijs van der Schot; Tomas Ekeberg; Janos Hajdu; Filipe Maia; Pierre Thibault; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Coherent diffractive X-ray imaging; flash X-ray imaging; lensless imaging; single particle imaging; cyanobacteria; phasing; image classification; substrate-free sample delivery; X-ray free-electron laser; XFEL; FEL; CDXI; CDI; CXI; FXI; Chemistry with specialization in Biophysics; Kemi med inriktning mot biofysik;

    Abstract : Imaging living cells at a resolution higher than the resolution of optical microscopy is a significant challenge. Fluorescence microscopy can achieve a degree of super-resolution via labeling cellular components with a fluorescent dye. READ MORE