Search for dissertations about: "methods in situ"

Showing result 1 - 5 of 527 swedish dissertations containing the words methods in situ.

  2. 1. In situ Sequencing : Methods for spatially-resolved transcriptome analysis

    Author : Marco Mignardi; Mats Nilsson; Xiaoliang Sunney Xie; Stockholms universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Padlock probes; rolling circle amplification; in situ sequencing; spatially-resolved transcriptomics; molecular diagnostics; Biochemistry; biokemi;

    Abstract : It is well known that cells in tissues display a large heterogeneity in gene expression due to differences in cell lineage origin and variation in the local environment at different sites in the tissue, a heterogeneity that is difficult to study by analyzing bulk RNA extracts from tissue. Recently, genome-wide transcriptome analysis technologies have enabled the analysis of this variation with single-cell resolution. READ MORE

  4. 2. Visualizing Interacting Biomolecules In Situ

    Author : Irene Weibrecht; Ola Söderberg; Ulf Landegren; Banafshe Larijani; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; proximity ligation; in situ PLA; padlock probe; rolling circle amplification; flow cytometry; in situ; single cell; single molecule; protein interaction; protein-DNA interaction; posttranslational modification; Molecular medicine; Molekylär medicin; Molekylär medicin; Molecular Medicine;

    Abstract : Intra- and intercellular information is communicated by posttranslational modifications (PTMs) and protein-protein interactions, transducing information over cell membranes and to the nucleus. A cells capability to respond to stimuli by several highly complex and dynamic signaling networks provides the basis for rapid responses and is fundamental for the cellular collaborations required in a multicellular organism. READ MORE

  5. 3. In situ measurements of radionuclide concentration in soil : An investigation into detector properties and methods

    Author : Johan Kastlander; Christoph Bargholtz; Mats Isaksson; Stockholms universitet; []
    Keywords : In situ gamma-ray spectrometry; LaBr3; CdTe; Cs-137; soil samples; fysik; Physics;

    Abstract : In case of a release of radioactive nuclides into the environment it is necessary to  have reliable methods to estimate the potential effect on people and the ecosystem. In this context the total activity deposited, the elemental composition and the depth distribution are of importance. READ MORE

  6. 4. Expression and Mutation Analyses of Candidate Cancer Genes In Situ

    Author : Sara Kiflemariam; Tobias Sjöblom; Fredrik Pontén; Mef Nilbert; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; cancer; in situ; in situ hybridization; padlock probes; mRNA; tyrosine kinases; tyrosine phosphatases; fusion transcripts;

    Abstract : Cancers display heterogeneity in genetic profiles of the individual cancer cells and in the composition of different malignant and non-malignant cell populations. Such intra-tumor heterogeneity plays a role in treatment response and the emergence of resistance to cancer therapies. READ MORE

  7. 5. In-situ TEM Probing of Nanomaterials

    Author : Magnus Hummelgård; Håkan Olin; Valeria Nicolosi; Mittuniversitetet; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; nanotechnology; in situ; TEM; probing; nanowires; nanoparticles; ink; Other engineering physics; Övrig teknisk fysik; Material physics with surface physics; Materialfysik med ytfysik; Condensed matter physics; Kondenserade materiens fysik;

    Abstract : Nanomaterials because of their small size, may have special properties unlikely to be seen in ordinary types of materials. Nanomaterials like nanotubes,nanowires and nanoparticles are best studied at the nanoscale, vital but also problematic. READ MORE