Search for dissertations about: "michaelis-menten kinetics"

Showing result 1 - 5 of 15 swedish dissertations containing the words michaelis-menten kinetics.

  2. 1. Mathematical modelling and analysis of the pyrosequencing reaction system

    Author : Anna Svantesson; Jeanette Hellgren Kotaleski; Anders Lansner; Pål Nyrén; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; apyrase; bioluminescence; DNA polymerase; DNA sequencing; enzyme kinetics; Klenow fragment; mathematical modelling; Michaelis-Menten; pyrosequencing; Computer science; Datalogi;

    Abstract : .... READ MORE

  4. 2. Targeting RNA by the Antisense Approach and a Close Look at RNA Cleavage Reaction

    Author : Jharna Barman; Lars Baltzer; Roger Strömberg; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Organic chemistry; mRNA targeting; antisense oligonucleotides; target affinity; RNase H; Michaelis-Menten kinetics; exo-nuclease stability; NMR; pH titration; alkaline hydrolysis; Organisk kemi;

    Abstract : This thesis summarizes the results of studies on two aspects of nucleic acids. Chemically modified antisense oligonucleotides (AONs) have been evaluated with regards to their suitability for mRNA targeting in an antisense approach (Paper I – III). READ MORE

  5. 3. Nucleotide Binding to Trimeric dUTPase: Studies on Recombinant Enzymes using Kinetic and Spectroscopic Methods

    Author : Johan Nord; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; C-terminus; dUTPase; EIAV; Escherichia coli; flexibility; Homo sapiens; kinetics; NMR; nucleotide metabolism; rate constants of lig-and binding; production of recombinant enzyme; dUTP; specificity; Biochemistry; Metabolism; Biokemi;

    Abstract : Recombinant dUTPase from the bacterium Escherichia coli, the retrovirus equine infectious anemia virus (EIAV) and Homo sapiens have been made available for physico-chemical characterisation. The protocols developed have also proven to be suitable for production of enzymes modified by isotope labelling or other methods. READ MORE

  6. 4. Molecular aspects of glutathione synthetase deficiency

    Author : Runa Vidar Njålsson; Karolinska Institutet; Karolinska Institutet; []
    Keywords : Glutathione synthetase; kinetics; cooperativity; substrate affinity; splice mutations; phenotype; genotype;

    Abstract : The tripeptide glutathione (GSH) is involved in several crucial pathways in the cell, for instance regulation of enzyme activity, DNA synthesis, and free radical scavenging. Synthesis of GSH takes place via two steps in the gamma-glutamyl cycle. READ MORE

  7. 5. Macroscopic Modeling of Metabolic Reaction Networks and Dynamic Identification of Elementary Flux Modes by Column Generation

    Author : Hildur Æsa Oddsdóttir; Anders Forsgren; Bernhard Palsson; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Metabolic Network; Optimization; Robust Optimization; Least-squares; Column Generation; Modeling; Algorithm; Elementary Flux Mode; Metabolic Flux Analysis; Chinese Hamster Ovary Cell; Amino Acid Metabolism; Tillämpad matematik och beräkningsmatematik; Applied and Computational Mathematics;

    Abstract : In this work an intersection between optimization methods and animal cell culture modeling is considered. We present optimization based methods for analyzing and building models of cell culture; models that could be used when designing the environment cells are cultivated in, i.e., medium. READ MORE