Search for dissertations about: "microfluidic superfusion"

Showing result 1 - 5 of 6 swedish dissertations containing the words microfluidic superfusion.

  2. 1. The Multifunctional Pipette. A Microfluidic Technology for the Biosciences

    Author : Alar Ainla; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; microfluidic superfusion; microfluidic temperature sensing.; microfluidic dilution; hydrodynamic flow confinement; microfluidic mixer; supported lipid membranes; single-cell analysis; Microfluidics; microfabrication; PDMS;

    Abstract : The theme of the work described in this thesis is the generation and application of liquid microenvironments in chemistry and bioscience using microfluidic devices. First, a computer controlled multi-stage dilution system to generate time-dependent chemical waves was developed, and its application was demonstrated on model biomembranes. READ MORE

  4. 2. On methods for probing structure and function of membrane proteins

    Author : Erik Jansson; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; proteomics; patch-clamp; mass spectrometry.; proteoliposomes; microfluidic superfusion; cholesterol; TRPV1; limited proteolysis; Membrane proteins; fluorescence;

    Abstract : The determination of structure–function relationships in membrane proteins plays a key role for the understanding of molecular mechanisms in biological systems, and may provide information on how these systems can be modulated by pharmaceuticals. The aim of the work described herein was to develop methods for studying structure and function of membrane proteins, ideally in combination. READ MORE

  5. 3. Patch-clamp studies of the GABAA receptor using microfluidic methods

    Author : Maria Millingen; Chalmers tekniska högskola; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; ion channel; microfluidics; GABAA receptor;

    Abstract : Ion channels are membrane proteins that passively transport ions. When activated, conformational changes within the protein lead to opening of a channel pore, and the flow of ions causes a change in the membrane potential. This is the fundamental process behind the generation and transduction of nerve impulses. READ MORE

  6. 4. Studies of ligand- and temperature-gated ion channels using microfluidic methods

    Author : Maria Millingen; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; solution exchange; desensitization; temperature-gated; ion channel; temperature control; ligand-gated; TRPV1; GABAA receptor; microfluidics;

    Abstract : This thesis describes the studies of the GABAA receptor, a ligand-gated ion channel, and the ligand- and temperature-gated ion channel TRPV1. The desensitization behavior of both of these proteins has been studied for different conditions, using a microfluidic device for superfusion. READ MORE

  7. 5. Microfluidics based techniques for electrophysiological studies of cells

    Author : Aikeremu Ahemaiti; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES;

    Abstract : This thesis work investigates the application of microfluidics to perform electrophysiological studies on cells, including investigations of the effect of cholesterol on the dynamic ion permeability of TRPV1 ion channels, and the application of a microfluidic device, the multifunctional pipette, in electrophysiological studies on brain slices. In the first part of this thesis, Chinese hamster ovary (CHO) cells overexpressing the TRPV1 ion channel were used in a dynamic ion permeability study, where the activation properties of the TRPV1 ion channel were investigated using the patch clamp technique after depletion of membrane cholesterol. READ MORE