Search for dissertations about: "multiplex protein quantification"

Showing result 1 - 5 of 9 swedish dissertations containing the words multiplex protein quantification.

2. 1. High-throughput protein analysis using mass spectrometry-based methods

   Author : Tove Boström; Sophia Hober; Thomas Laurell; Ole N. Jensen; KTH; []
   Keywords : Proteomics; mass spectrometry; affinity proteomics; immunoenrichment; immunoprecipitation; IMAC; screening; protein production; protein purification; ISET; quantification; SILAC; stable isotope standard; antibody validation; Bioteknologi; Biotechnology;

   Abstract : In the field of proteomics, proteins are analyzed and quantified in high numbers. Protein analysis is of great importance and can for example generate information regarding protein function and involvement in disease. Different strategies for protein analysis and quan- tification have emerged, suitable for different applications. READ MORE

4. 2. Multiplexed absolute quantification of blood plasma proteins using targeted proteomics

   Author : David Kotol; Mathias Uhlén; Edfors Fredrik; Roman Zubarev; KTH; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; mass spectrometry; targeted proteomics; stable isotope standards; recombinant protein standards; absolute quantification; blood plasma; multiplex analysis; plasma profiling; Biotechnology; Bioteknologi;

   Abstract : Proteins are the molecular building blocks of all living organisms. They are the functioning actors in metabolism and communication, and they give specific architecture and purpose to all tissues. Their abundance is dynamic and can be interpreted and associated to different physiological states or diseases. READ MORE

5. 3. Solid-phase Proximity Ligation Assays : High-performance and multiplex protein analyses

   Author : Spyros Darmanis; Masood Kamali-Moghaddam; Ulf Landegren; Mathias Uhlen; Uppsala universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; proximity ligation assay; multiplexed; biomarkers; solid-phase; microparticles; cancer; sequencing; Molecular biology; Molekylärbiologi; Biology with specialization in Molecular Biotechnology; Biologi med inriktning mot molekylär bioteknik;

   Abstract : Protein biomarkers circulating in blood hold the promise of improved diagnosis, prognosis and follow-up of treatment of disease via minimally invasive procedures. For the discovery and validation of such biomarkers, methods are needed that can facilitate parallel, highly specific and in-depth analysis of the blood proteome. READ MORE

6. 4. Microfluidic Methods for Protein Microarrays

   Author : Michael Hartmann; Johan Roeraade; Mike Taussig; KTH; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Analytical chemistry; Analytisk kemi;

   Abstract : Protein microarray technology has an enormous potential for in vitro diagnostics (IVD)1. Miniaturized and parallelized immunoassays are powerful tools to measure dozens of parameters from minute amounts of sample, whilst only requiring small amounts of reagent. READ MORE

7. 5. Myeloid-Derived Suppressor Cells and Other Immune Escape Mechanisms in Chronic Leukemia

   Author : Lisa Christiansson; Angelica S I Loskog; Ulla Olsson-Strömberg; Andreas Lundqvist; Uppsala universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; chronic myeloid leukemia; myeloid-derived suppressor cells; sCD25; tyrosine kinase inhibitors; multiplex protein quantification; Medical Science; Medicinsk vetenskap;

   Abstract : Chronic myeloid leukemia (CML) is characterized by the Philadelphia chromosome, a minute chromosome that leads to the creation of the fusion gene BCR/ABL and the transcription of the fusion protein BCR/ABL in transformed cells. The constitutively active tyrosine kinase BCR/ABL confers enhanced proliferation and survival on leukemic cells. READ MORE