Search for dissertations about: "pichia pastoris fermentation"

Showing result 1 - 5 of 6 swedish dissertations containing the words pichia pastoris fermentation.

  2. 1. Expression and Mutagenesis studies of Candida antactica lipase B

    Author : Johanna C. Rotticci-Mulder; KTH; []
    Keywords : Protein expression; Candida antarctica lipase B; Pichia pastoris; site-directed mutagenesis; fermentation; selection;

    Abstract : Recombinant Candida antarctica lipase B was successfullyproduced in the methylotropic yeast Pichia pastoris. Thespecific activities of Candida antarctica lipase B produced inPichia pastoris and commercial Candida antarctica lipase B fromNovozymes were the same. In shake-flask cultivations theexpression levels were about 25 mg L-1. READ MORE

  4. 2. Production and engineering of a xyloglucan endo-transglycosylase from Populus tremula x tremuloides

    Author : Maria Henriksson; Tuula Teeri; KTH; []
    Keywords : LANTBRUKSVETENSKAPER; AGRICULTURAL SCIENCES; xyloglucan endo-transglycosylase; retaining glycoside hydrolase; glycosynthase; Pichia pastoris; fed-batch fermentation; expanded-bed adsorption; Plant biotechnology; Växtbioteknik;

    Abstract : The aim of this work was to develop a production process for the enzyme xyloglucan endo-transglycosylase from Populus tremula x tremuloides (PttXET16-34). The natural transglycosylating activity of this enzyme has previously been employed in a XET-Technology. READ MORE

  5. 3. Yeasts as cellular factories: Saccharomyces cerevisiae strains with improved inhibitor-tolerance to furaldehydes via a mutant alcohol dehydrogenase- and- Human ameloblastin production in Pichia pastoris

    Author : Boaz Laadan; Teknisk mikrobiologi; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY;

    Abstract : Several yeast species, usually engineered by humans in order to produce compounds of biotechnological interests, are currently used as cellular factories. This thesis presents two main themes. READ MORE

  6. 4. Four carbon oxychemicals from renewable resources

    Author : Adam Marschall Jaros; Christian Andersson; Luleå tekniska universitet; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Biokemisk processteknik; Biochemical Process Engineering;

    Abstract : Butanol, butyric acid and butyraldehyde are important 4-carbon oxychemicals typically generated from petro-chemical sources. All have significant markets in the food industry either for direct use as flavorings or as chemical feedstocks for generating butyric acid and butyraldehyde derived flavour compounds. READ MORE

  7. 5. Biorefining of lignocellulose : Detoxification of inhibitory hydrolysates and potential utilization of residual streams for production of enzymes

    Author : Adnan Cavka; Leif J. Jönsson; John N. Saddler; Umeå universitet; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY;

    Abstract : Lignocellulosic biomass is a renewable resource that can be utilized for the production of biofuels, chemicals, and bio-based materials. Biochemical conversion of lignocellulose to advanced biofuels, such as cellulosic ethanol, is generally performed through microbial fermentation of sugars generated by thermochemical pretreatment of the biomass followed by an enzymatic hydrolysis of the cellulose. READ MORE