Search for dissertations about: "plasmid copy number"

Showing result 1 - 5 of 11 swedish dissertations containing the words plasmid copy number.

2. 1. Molecular mechanism of plasmid copy number control in Yersinia

   Author : Tifaine Héchard; Helen Wang; Javier Pizarro-Cerda; Uppsala universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Yersinia pseudotuberculosis; plasmid copy number; T3SS; infection; ddPCR; Mikrobiologi; Microbiology;

   Abstract : The ability of pathogenic bacteria to cause disease depends on various virulence mechanisms. The three pathogenic species of Yersinia use a type III secretion system (T3SS) to translocate effector proteins into host cells and disrupt the immune system. This T3SS is encoded on a 70kb, low-copy, virulence plasmid. READ MORE

4. 2. CopA and CopT: The Perfect RNA Couple

   Author : Jacoba G. Slagter-Jäger; Gerhart Wagner; Wolfgang Nellen; Uppsala universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Microbiology; antisense RNA; plasmid; U-turn; four-way junction; RNA-RNA interaction; Hfq; Mikrobiologi; Microbiology; Mikrobiologi; mikrobiologi; Microbiology;

   Abstract : Antisense RNAs regulate gene expression in many bacterial systems. The best characterized examples are from prokaryotic accessory elements such as phages, plasmids and transposons. Many of these antisense RNAs have been identified as plasmid copy number regulators where they regulate the replication frequency of the plasmid by negative feedback. READ MORE

5. 3. The stochastic nature of intracellular control circuits

   Author : Johan Paulsson; Uppsala universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Cell and molecular biology; Stochastic; mesoscopic; noise; gene; plasmid; metabolic; regulation; control; Cell- och molekylärbiologi; Cell and molecular biology; Cell- och molekylärbiologi; molekylärbiologi; Molecular Biology;

   Abstract : Chemical reactions are probabilistic by nature, causing random fluctuations inconcentrations to emerge spontaneously. In conventional (macroscopic) kinetics this internal noise is ignored under the assumption that average copy numbers are so high that fluctuations are negligible in comparison. READ MORE

6. 4. Physiology of Saccharomyces cerevisiae Producing Recombinant Insulin in Continuous Culture

   Author : Ali Kazemi Seresht; Chalmers tekniska högskola; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; unfolded protein response; Keywords: heterologous protein production; prolonged chemostat; transcriptome; insulin analogue precursor; metabolic adaptation; URA3d; plasmid copy number; promoter truncation; high cell density cultivation; TPI1;

   Abstract : The yeast Saccharomyces cerevisiae has widely been used as a host for the production of heterologous proteins. High-level production of heterologous proteins is likely to impose a metabolic burden on the host cell and can thus affect various aspects of cellular physiology. READ MORE

7. 5. The Involvement of the structures of antisense RNA, target RNA, and their complexes in plasmid R1 copy number regulation

   Author : Charlotta Ingvoldstad; Uppsala universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Cell and molecular biology; Cell- och molekylärbiologi; Cell and molecular biology; Cell- och molekylärbiologi; mikrobiologi; Microbiology;

   Abstract : Plasmid R1 is a low-copy-number plasmid which belongs to the incompatibility group FII. R1 regulates its own copy number. Initiation of replication of plasmid R1 requires the binding of the RepA protein to oriR1. RepA acts in cis and has to be synthesized de novo for each round of replication. READ MORE