Search for dissertations about: "protein solubility"

Showing result 1 - 5 of 53 swedish dissertations containing the words protein solubility.

2. 1. Protein isolation from herring (Clupea harengus) using the pH-shift process - Protein yield, protein isolate quality and removal of food contaminants

   Author : Sofia Marmon; Chalmers tekniska högskola; []
   Keywords : LANTBRUKSVETENSKAPER; AGRICULTURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; precipitation; acid; gelation; in vitro digestion; solubilization; herring; protein; salt solubility; pH; lipids; alkaline; transmission electron microscopy; dioxins;

   Abstract : Herring (Clupea harengus) contain valuable proteins but is difficult to process into high-quality foods due to its small size and high content of bones, heme-proteins and lipids. Herring is among the most abundant fish species in the world, but is currently utilized largely for fish meal and oil production. READ MORE

4. 2. Protein production, characterization and structure determination in structural genomics

   Author : Esmeralda A. Woestenenk; Torleif Härd; Göran Karlsson; KTH; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biology; protein production; protein solubility; fusion tags; nuclear magnetic resonance; structure determination; Biologi; Biology; Biologi;

   Abstract : This thesis covers the process from expression of a heterologous gene in Escherichia coli to structure determination of a protein by nuclear magnetic resonance (NMR) spectroscopy. The first part concerns structural genomics-related parallel screening studies on the effect of fusion tags (in particular the His tag) on protein solubility and the use of fusion tags in fast, parallel purification protocols intended for initial biophysical characterization of human proteins produced in E. READ MORE

5. 3. Strategies to improve the aging, barrier and mechanical properties of chitosan, whey and wheat gluten protein films

   Author : Idoia Olabarrieta; Mikael Hedenqvist; Jose Maria Lagaron; KTH; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Chemistry; biodegradable polymers; chitosan; whey protein; wheat gluten; poly ε-caprolactone ; montmorillonite; food packaging; permeability; mechanical properties; aging; pH; solubility; migration; solution casting.; Kemi; Chemistry; Kemi;

   Abstract : Chitosan, Whey Protein Isolate (WPI) and vital wheat gluten (WG) are three biomaterials that have quite promising properties for packaging purposes. They have good film forming properties and good gas barrier properties in dry conditions. READ MORE

6. 4. Protein production and purification in structural genomics

   Author : Martin Hammarström; Per-Åke Nygren; Torleif Härd; Helena Berglund; Herman van Tilbeurgh; KTH; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Recombinational cloning; Escherichia coli; gene expression; fusion proteins; solubility; circular dichroism; nuclear magnetic resonance; Structural biochemistry; Strukturbiokemi;

   Abstract : The number of gene products available for structural and functional study is increasing at an unprecedented rate as a result of the successful whole genome sequencing projects. Systematic structure determination of proteins on a genomic scale, called structural genomics, can significantly contribute to the field of protein science and to functional annotation of newly identified genes. READ MORE

7. 5. Characterization of Antigenic Properties and High Throughput Protein Purification

   Author : Johanna Steen; Sophia Hober; Mats Ohlin; KTH; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; high throughput; protein purification; sample preparation; antigen solubility; immunogenicity; epitope mapping; antigen design; Bioengineering; Bioteknik; SRA - Molecular Bioscience; SRA - Molekylär biovetenskap;

   Abstract : To understand the cellular processes, knowledge of the localization and function of proteins are essential. There are several high throughput ventures examining the human proteome. However, there are some bottlenecks in these ventures. For example the production and expression of soluble proteins for analysis. READ MORE