Search for dissertations about: "proteoliposome assay"

Found 4 swedish dissertations containing the words proteoliposome assay.

  2. 1. Quantitation, Purification and Reconstitution of the Red Blood Cell Glucose Transporter GLUT1

    Author : Shusheng Zuo; Bengt Mannervik; Bengt Persson; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Cholesterol; Cytochalasin B; Glucose; GLUT1; Hummel and Dreyer analysis; Immobilization; PEG 5000 -DSPE; Biomembrane; Proteoliposome; Quantitative frontal affinity chromatography; Red blood cell streptavidin-biotin immobilization; Sulfhydryl affinity chromatography; The modified micro-Bradford CaPE assay; MALDI-ToF-MS; Biokemi; Biochemistry; Biokemi;

    Abstract : The human glucose transporter GLUT1 facilitates glucose to be accumulated on the other side of the cell membrane. The functional state of GLUT1 is uncertain due to diversity of the reports. In this thesis, the activity of red blood cell GLUT1 was extensively studied to further characterize this protein. READ MORE

  4. 2. Plant aquaporin regulation: Structural and functional studies using diffraction and scattering techniques

    Author : Michael Järvå; Göteborgs universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; X-ray Crystallography; Membrane protein; Plasma membrane intrinsic protein; Aquaporin; pH gating; mercury; proteoliposome assay; water transport; SANS; SAXS; WAXS; nanodisc; liposome;

    Abstract : Water is the basis for life as we know it. It is only logical then that all organisms have evolved specialized proteins, aquaporins, which regulate water flow across their membranes. Plants, which are immobile, depend more on their environment and also use water flows to move, to breathe, and to grow. READ MORE

  5. 3. The molecular basis for substrate recognition and gating in sugar transporters

    Author : Sarah McComas; David Drew; Philip Biggin; Stockholms universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; membrane transport; sugar transporter; simulations; lipids; antimalarial drugs; biokemi; Biochemistry;

    Abstract : Sugar is a vital sustenance for most forms of life. For a cell to take up sugar, specialized transport proteins embedded into the membrane bilayer known as sugar porters, are required. READ MORE

  6. 4. Design, synthesis and evaluation of bacterial sialic acid uptake inhibitors

    Author : Tiago Bozzola; Centrum för analys och syntes; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Sialic Acid; antibacterial agents; Medicinal Chemistry; Synthesis; uptake inhibitors;

    Abstract : Antibiotic resistance is a major threat for our society and finding novel antibacterial therapies is of greatimportance. In this thesis, we investigate bacterial sialic acid uptake inhibitors as a novel antibacterial approach.Bacteria do not biosynthesise sialic acid and therefore harvest it from the host. READ MORE