Search for dissertations about: "qRT-PCR"

Showing result 1 - 5 of 45 swedish dissertations containing the word qRT-PCR.

  2. 1. Real-time PCR for diagnosis of botulism and quantification of neurotoxin gene expression in Clostridium botulinum

    Author : Ingrid Artin; Avdelningen för medicinsk mikrobiologi; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; detection; qRT-PCR; Clostridium botulinum; molecular methods;

    Abstract : The objectives of the work presented in this thesis have been to develop molecular methods for (i) detection of Clostridium botulinum in food and clinical samples, and (ii) monitoring the expression of the neurotoxin gene, cnt, during different growth phases and under different growth conditions. For the diagnosis of botulism, a real-time PCR-based method including an internal amplification control for C. READ MORE

  4. 2. A Molecular Approach for Investigation of the Prevalence and Neurotoxin Formation of Clostridium botulinum in Food Safety Assessment

    Author : Maria Lövenklev; Teknisk mikrobiologi; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; neurotoxin gene expression; neurotoxin; food safety assessment; food preservation; Clostridium botulinum; enrichment PCR; Food and drink technology; qRT-PCR; Livsmedelsteknik; prevalence;

    Abstract : In the development of new food products, more knowledge is needed about the occurrence and quantity of food-borne pathogens in the food chain to be able to implement effective control measures and assure food safety. In addition, improved understanding of how environmental factors and food preservatives effect the microbial virulence expression in foods will enable the formulation of new strategies for food preservation and risk assessment. READ MORE

  5. 3. Dynamics of Human Leukocyte Antigen-D Related expression in bacteremic sepsis

    Author : Sara Cajander; Jan Källman; Kristoffer Strålin; Bo Söderquist; Per Åkesson; Örebro universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; monocyte HLA-DR; sepsis; immunosuppression; bloodstream infection; HLA-DRA; CIITA; qRT-PCR;

    Abstract : Monocytic human leukocyte antigen-D related (mHLA-DR) expression determined by flow cytometry has been suggested as a biomarker of sepsisinduced immunosuppression.In order to facilitate use of HLA-DR in clinical practice, a quantitative real-time PCR technique measuring HLA-DR at the transcription level was developed and evalutated. READ MORE

  6. 4. Stress and the Offspring : Adaptive Transgenerational Effects of Unpredictability on Behaviour and Gene Expression in Chickens (Gallus gallus)

    Author : Daniel Nätt; Per Jensen; Berry Spruijt; Linköpings universitet; []
    Keywords : Chicken; Red junglefowl; Domestication; Epigenetics; Transgenerational; Inheritance; Gene expression; Microarray; qRT-PCR; Immunogenetics; Immunoglobulin; Estradiol; Steriod hormones; Prenatal stress; Epigenetic inheritance; Adaptation; Stress; Behaviour; Ethology of domestic animals; Husdjurens etologi;

    Abstract : Environmental stress has shown to affect both the exposed individuals and the development of their offspring. Generally, it is thought that the stressed organism responds to stress by trying to adapt to it. READ MORE

  7. 5. Molecular interactions between quinoa, the biocontrol agent Trichoderma and the pathogen Peronospora variabilis

    Author : Oscar Miguel Rollano Penaloza; Molekylär cellbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Chenopodium quinoa; Peronospora variabilis; Trichoderma harzianum; Molecular interactions; qRT-PCR; RNA-seq; Plant growth promotion; Chenopodium quinoa; Peronospora variabilis; Trichoderma harzianum; RNA-seq; qRT-PCR; molekylära interaktioner;

    Abstract : Plants have developed complex molecular mechanisms to recognize and respond to the different microorganisms present in their surroundings. The most studied response mechanisms are plant defense responses. These defense response mechanisms share many similar components with the plant response mechanisms to beneficial microbes. READ MORE