Search for dissertations about: "quantitative PCR"

Showing result 1 - 5 of 238 swedish dissertations containing the words quantitative PCR.

  2. 1. Diagnostic PCR for the Detection of Yersinia enterocolitica and Salmonella in the Food-Chain: Reliability of PCR Performance

    Author : Rickard Knutsson; Teknisk mikrobiologi; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Livsmedelsteknik; Food and drink technology; Kemiteknik och kemisk teknologi; Chemical technology and engineering; real-time PCR; YPCE medium; Enrichment PCR; DNA polymerases; PCR inhibitors; pre-PCR processing; Salmonella; Diagnostic PCR; Yersinia enterocolitica; Microbiology; bacteriology; virology; mycology; Mikrobiologi; bakteriologi; virologi; mykologi;

    Abstract : This thesis deals with the methodological advances of diagnostic PCR including reliability of PCR and pre-PCR processing of food and feed samples. Diagnostic PCR has been greatly improved by the introduction of the second generation of PCR, so-called real-time PCR. READ MORE

  4. 2. Quantitative detection of bacterial DNA in whole blood in bloodstream infection

    Author : Ingrid Ziegler; Kristoffer Strålin; Paula Mölling; Magnus Rasmussen; Örebro universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Bloodstream infection; bacteremia; sepsis; DNA load; quantitative PCR; droplet digital PCR; 16S rDNA;

    Abstract : This thesis aims to increase the knowledge on how quantitative PCR can be used in the diagnostics of bloodstream infections, with an emphasis on quantitative elements.In Papers I and II, we evaluated quantitative data from two commercial PCR tests for pathogen detection directly in blood, Magicplex Sepsis (I) and SeptiFast (II), from patients with suspected sepsis. READ MORE

  5. 3. Pre-PCR Processing Strategies for Quantitative Detection of Food-Borne Pathogens using Real-Time PCR

    Author : Petra Wolffs; Teknisk mikrobiologi; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Livsmedelsteknik; Food and drink technology; DNA polymerase; sample treatment; pre-PCR processing; real-time PCR; Food-borne pathogens;

    Abstract : Since the development of real-time PCR, the technology has been widely applied in the field of diagnostics. In comparison with conventional PCR it has opened up the possibility of accurate quantification of microorganisms in clinical, environmental and food samples. READ MORE

  6. 4. Development of Real-Time PCR Based Methods for Detection of Viruses and Virus Antibodies

    Author : Amal Elfaitouri; Jonas Blomberg; Helene Norder; Uppsala universitet; []
    Keywords : Microbiology; Real-time PCR; broadly targeted PCR; degenerated PCR; rTth DNA polymerase; thermolabile UNG; QPIA; IgM; Mikrobiologi;

    Abstract : Quantitative real-time PCR (QPCR) technology has been very useful for diagnosis of viral diseases. QPCR has recently reached a level of sensitivity, simplicity, and reproducibility which allows a large number of samples to be screened rapidly, make it a suitable tool for the clinical virology diagnostics. READ MORE

  7. 5. Transcriptional biomarkers of toxicity – powerful tools or random noise? : An applied perspective from studies on bivalves

    Author : Gustaf Magnus Oskar Ekelund Ugge; Olof Berglund; Annie Jonsson; Helena Westerdahl; Mikael Ejdebäck; Bruno Nunes; Högskolan i Skövde; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Biomarkers; Bivalves; Bivalvia; Ecotoxicology; Environmental toxicology; Environmental risk assessment; RT-qPCR; qPCR; Quantitative PCR; Systematic review; Meta-analysis; Dose-response relationships; Concentration-response relationships; Heavy metals; Metals; General toxicity; Copper; Duck mussel; Anodonta anatina; Freshwater; Gene transcription; Biomarkörer; Musslor; Bivalvia; Ekotoxikologi; Miljötoxikologi; Miljöriskbedömning; RT-qPCR; qPCR; Kvantitativ PCR; Systematic review; Metaanalys; Dos-responssamband; Koncentration-responssamband; Tungmetaller; Metaller; Allmän toxicitet; Koppar; Allmän dammussla; Anodonta anatina; Sötvatten; Gentranskription; Ekologisk modellering; Ecological Modelling Group; Biomarkörer; Musslor; Bivalvia; Ekotoxikologi; Miljötoxikologi; Miljöriskbedömning; RT-qPCR; qPCR; Kvantitativ PCR; Systematic review; Metaanalys; Dos-responssamband; Koncentration-responssamband; Tungmetaller; Metaller; Allmän toxicitet; Koppar; Allmän dammussla; Anodonta anatina; Sötvaten; Gentranskription; Biomarkers; Bivalves; Ecotoxicology; Environmental toxicology; Environmental risk assessment; Quantitative PCR; Meta-analysis; Dose-response relationships; Concentration-response relationships; Heavy metals; Metals; General toxicity; Copper; Duck mussel; Freshwater; Gene transcription;

    Abstract : Aquatic organisms are constantly at risk of being exposed to potentially harmful chemical compounds of natural or anthropogenic origin. Biological life can for instance respond to chemical stressors by changes in gene expression, and thus, certain gene transcripts can potentially function as biomarkers, i.e. READ MORE